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Radiolabelling of Super-diffuse Amyloid-b Deposition with Popularly Used Amyloid Imaging Ligands in Postmortem Brain of Alzheimer’s Disease
https://repo.qst.go.jp/records/71737
https://repo.qst.go.jp/records/717374776b0fd-8f9c-4f34-9c65-151c7020863a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-02-19 | |||||
| タイトル | ||||||
| タイトル | Radiolabelling of Super-diffuse Amyloid-b Deposition with Popularly Used Amyloid Imaging Ligands in Postmortem Brain of Alzheimer’s Disease | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
BinJi
× BinJi× Chun-Jen, Chen× Bando, Kazunori× Ashino, Hiroki× Ono, Maiko× Minamihisamatsu, Takeharu× Suhara, Tetsuya× Higuchi, Makoto× Ji, Bin× Chun-Jen, Chen× Ono, Maiko× Minamihisamatsu, Takeharu× Suhara, Tetsuya× Higuchi, Makoto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background/Purpose: We have successfully visualized amyloid- (A) deposition in a mouse model of Alzheimer’s disease (AD) with the aid of newly developed amyloid imaging tracer 123/125I-DRM106, and suggested different binding site in aggregated A for this compound with popularly used amyloid imaging tracers such as PiB (Pittsburgh Compound B) in our previous study (J Nucl Med; 2015). In the present study, we have investigated the autoradiogram of radioiodinated DRM106 and other several amyloid imaging tracers in postmortem AD brain to examine the possibility of distinguishing different types of A deposits by combination of these tracers. Materials & Methods: The AD brain sections containing the hippocampal and parahippocampal regions were incubated with radioactive tracers (11C-PiB; 5nM, 3H-AZD2184; 5nM, 125I-DRM106; 5nM) for 60 minutes, followed by consequent processes of autoradiographic analysis. Amyloid deposition was determined by fluorescent counterstaining with FSB and immunohistochemical staining with anti-A antibody (6E10). Results: Autoradiographic images showed there is greatly difference in the feature of binding to brain subregions between tested tracers. There were greatly abundant binding sites for 11C-PiB and 3H-AZD2184, but not for 125I-DRM106, in the presubiculum of AD brain, where, abundant super-diffuse A deposits reacted with 6E10, was FSB-negative by fluorescent microscopic observation. These data indicate preferentiality of DRM106 to highly maturated A deposits compared with PiB and AZD2184. Conclusions: The present result reveal possible contribution of those minor A pathology, such as super-diffuse plaques, to binding sites for these popularly used tracers, and it might be distinguishable by combination scans with different type amyloid imaging tracers such as PiB/AZD2184 and DRM106. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Japan-China Nuclear Medicine joint symposium in Okinawa | |||||
| 発表年月日 | ||||||
| 日付 | 2015-03-15 | |||||
| 日付タイプ | Issued | |||||
