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The Induction of Cycloloxygenase-2(COX-2) Expression Responding to Ischemic Neuronal Injury and PET Imaging with Two Radio-labeled First-generation COX-2 Selective Inhibitors
https://repo.qst.go.jp/records/71606
https://repo.qst.go.jp/records/716062b813759-0a0b-4431-8e7d-1e6fca3134ae
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-04-20 | |||||
| タイトル | ||||||
| タイトル | The Induction of Cycloloxygenase-2(COX-2) Expression Responding to Ischemic Neuronal Injury and PET Imaging with Two Radio-labeled First-generation COX-2 Selective Inhibitors | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ji, Bin
× Ji, Bin× Zhang, Ming-Rong× Kaneko, Hiroyuki× Kumata, Katsushi× Seki, Chie× Ono, Maiko× Tokunaga, Masaki× Minamihisamatsu, Takeharu× Higuchi, Makoto× Suhara, Tetsuya× et.al× 季 斌× 張 明栄× 金子 博之× 熊田 勝志× 関 千江× 小野 麻衣子× 徳永 正希× 南久松 丈晴× 樋口 真人× 須原 哲也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: To clarify whether imaging for cyclooxygenase-2(COX-2) is potential for monitoring pathological advance in ischemic diseases, and labeled COX-2 selective inhibitors are available as positron emission tomography (PET) tracers for COX-2 imaging. Methods: We have investigated the expression of COX-2 in normal and ischemic mouse brain with an original anti-COX-2 antibody with high specificity against murine COX-2 molecule and performed in-vivo living imaging and in-vitro autoradiographic analysis with two radio-synthesized first-generation COX-2 selective inhibitors ([11C]celecoxib) and [11C]rofecoxib), in normal and ischemic mouse brains Results: The immunohistochemical analysis showed the expression of COX-2 was exclusively localized in neurons with the most abundant amount in cerebellum and brainstem in normal mouse brain, and such expression was detectable by in-vitro autoradiographic analysis with [11C]rofecoxib, not [11C]celecoxib. In-vivo imaging with both of these two PET tracers failed to detect COX-2 expression despite their excellent brain permeability. The hypoperfusion-induced ischemia caused overt necrotic neuron death accompanied with gliosis in hippocampus, where significant induction of COX-2 was detected exclusively in injured neuron soma and synapse-like structure. The binding of [11C]rofecoxib was also increased in injured hippocampus compared to uninjured corresponding region. Conclusions: COX-2 is suitable biomarker for monitoring progressive ischemic injury and developing PET tracers for COX-2 imaging with COX-2 selective inhibitors is feasible for this purpose, although in-vivo living imaging requires further development of PET tracer with higher affinity than [11C]celecoxib and [11C]rofecoxib. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | BRAIN'13 and BRAINPET'13 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-23 | |||||
| 日付タイプ | Issued | |||||
