@misc{oai:repo.qst.go.jp:00071606,
 author = {Ji, Bin and Zhang, Ming-Rong and Kaneko, Hiroyuki and Kumata, Katsushi and Seki, Chie and Ono, Maiko and Tokunaga, Masaki and Minamihisamatsu, Takeharu and Higuchi, Makoto and Suhara, Tetsuya and et.al and 季 斌 and 張 明栄 and 金子 博之 and 熊田 勝志 and 関 千江 and 小野 麻衣子 and 徳永 正希 and 南久松 丈晴 and 樋口 真人 and 須原 哲也},
 month = {May},
 note = {Objectives: To clarify whether imaging for cyclooxygenase-2(COX-2) is potential for monitoring pathological advance in ischemic diseases, and labeled COX-2 selective inhibitors are available as positron emission tomography (PET) tracers for COX-2 imaging. 
Methods: We have investigated the expression of COX-2 in normal and ischemic mouse brain with an original anti-COX-2 antibody with high specificity against murine COX-2 molecule and performed in-vivo living imaging and in-vitro autoradiographic analysis with two radio-synthesized first-generation COX-2 selective inhibitors ([11C]celecoxib) and [11C]rofecoxib), in normal and ischemic mouse brains 
Results: The immunohistochemical analysis showed the expression of COX-2 was exclusively localized in neurons with the most abundant amount in cerebellum and brainstem in normal mouse brain, and such expression was detectable by in-vitro autoradiographic analysis with [11C]rofecoxib, not [11C]celecoxib. In-vivo imaging with both of these two PET tracers failed to detect COX-2 expression despite their excellent brain permeability. The hypoperfusion-induced ischemia caused overt necrotic neuron death accompanied with gliosis in hippocampus, where significant induction of COX-2 was detected exclusively in injured neuron soma and synapse-like structure. The binding of [11C]rofecoxib was also increased in injured hippocampus compared to uninjured corresponding region.
Conclusions: COX-2 is suitable biomarker for monitoring progressive ischemic injury and developing PET tracers for COX-2 imaging with COX-2 selective inhibitors is feasible for this purpose, although in-vivo living imaging requires further development of PET tracer with higher affinity than [11C]celecoxib and [11C]rofecoxib., BRAIN'13 and BRAINPET'13},
 title = {The Induction of Cycloloxygenase-2(COX-2) Expression Responding to Ischemic Neuronal Injury and PET Imaging with Two Radio-labeled First-generation COX-2 Selective Inhibitors},
 year = {2013}
}