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siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール
https://repo.qst.go.jp/records/71355
https://repo.qst.go.jp/records/71355da3a4c07-873c-45e6-9fab-12d36aa35b22
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-11-14 | |||||
| タイトル | ||||||
| タイトル | siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
村山, 周平
× 村山, 周平× 加藤, 大× 村山 周平× 加藤 大 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although the use of small interfering RNA(siRNA)is a promising technique for gene expression regulation,spatiotemporal control of the effects of siRNA must be achieved if the technique is to be safe and practical. Here, we report a method for spatiotemporal and quantitative regulation of gened with nanoparticles containing siRNA. The siRNA is encapsulated in photodegradable nanoparticles that are internalized to SKOV3-Luc cells,where the siRNA is released from the nanoparticled by UV irradiation for 30s. The encapsulated siRNA only shouws no gene-silencing effects,but release of the siRNA upon UV radiation leads to sequence-specific silencing of the luciferase gene in the cells. By using 1000 pmol encapsulated siRNA, more strong inhibition was occurred than 100 pmol siRNA. These results indicate that photodegradable siRNA-containing nanoparticles can be useful for time-and space-dependent and quantitative regulation of gene expression in cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第24回クロマトグラフィー科学会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-11-13 | |||||
| 日付タイプ | Issued | |||||
