{"_buckets": {"deposit": "761ca6db-56a4-41ad-93fd-176542014dbf"}, "_deposit": {"created_by": 1, "id": "71355", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "71355"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00071355", "sets": ["28"]}, "author_link": ["701612", "701611", "701614", "701613"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2013-11-13", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Although the use of small interfering RNA(siRNA)is a promising technique for gene expression regulation,spatiotemporal control of the effects of siRNA must be achieved if the technique is to be safe and practical. Here, we report a method for spatiotemporal and quantitative regulation of gened with nanoparticles containing siRNA. The siRNA is encapsulated in photodegradable nanoparticles that are internalized to SKOV3-Luc cells,where the siRNA is released from the nanoparticled by UV irradiation for 30s. The encapsulated siRNA only shouws no gene-silencing effects,but release of the siRNA upon UV radiation leads to sequence-specific silencing of the luciferase gene in the cells. By using 1000 pmol encapsulated siRNA, more strong inhibition was occurred than 100 pmol siRNA. These results indicate that photodegradable siRNA-containing nanoparticles can be useful for time-and space-dependent and quantitative regulation of gene expression in cells.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要（会議名, 開催地, 会期, 主催者等）", "attribute_value_mlt": [{"subitem_description": "第24回クロマトグラフィー科学会議", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "村山, 周平"}], "nameIdentifiers": [{"nameIdentifier": "701611", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "加藤, 大"}], "nameIdentifiers": [{"nameIdentifier": "701612", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "村山 周平", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701613", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "加藤 大", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "701614", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/71355", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-11-14"}, "publish_date": "2013-11-14", "publish_status": "0", "recid": "71355", "relation": {}, "relation_version_is_last": true, "title": ["siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール"], "weko_shared_id": -1}