{"created":"2023-05-15T14:52:15.675334+00:00","id":71355,"links":{},"metadata":{"_buckets":{"deposit":"761ca6db-56a4-41ad-93fd-176542014dbf"},"_deposit":{"created_by":1,"id":"71355","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71355"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071355","sets":["10:28"]},"author_link":["701612","701611","701614","701613"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-11-13","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although the use of small interfering RNA(siRNA)is a promising technique for gene expression regulation,spatiotemporal control of the effects of siRNA must be achieved if the technique is to be safe and practical. Here, we report a method for spatiotemporal and quantitative regulation of gened with nanoparticles containing siRNA. The siRNA is encapsulated in photodegradable nanoparticles that are internalized to SKOV3-Luc cells,where the siRNA is released from the nanoparticled by UV irradiation for 30s. The encapsulated siRNA only shouws no gene-silencing effects,but release of the siRNA upon UV radiation leads to sequence-specific silencing of the luciferase gene in the cells. By using 1000 pmol encapsulated siRNA, more strong inhibition was occurred than 100 pmol siRNA. These results indicate that photodegradable siRNA-containing nanoparticles can be useful for time-and space-dependent and quantitative regulation of gene expression in cells.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第24回クロマトグラフィー科学会議","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"村山, 周平"}],"nameIdentifiers":[{"nameIdentifier":"701611","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤, 大"}],"nameIdentifiers":[{"nameIdentifier":"701612","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村山 周平","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"701613","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤 大","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"701614","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-11-14"},"publish_date":"2013-11-14","publish_status":"0","recid":"71355","relation_version_is_last":true,"title":["siRNA内包光分解性ナノ粒子ゲルを用いた遺伝子発現強度のコントロール"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:52:13.235907+00:00"}