WEKO3
アイテム
Distinct Effects of Carbon Ion Beam and X-ray on Putative Liver Cancer Stem Cell In Vitro and In Vivo
https://repo.qst.go.jp/records/71171
https://repo.qst.go.jp/records/711715b359e11-5f2c-48d7-aa32-240873d4eedd
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-06-24 | |||||
| タイトル | ||||||
| タイトル | Distinct Effects of Carbon Ion Beam and X-ray on Putative Liver Cancer Stem Cell In Vitro and In Vivo | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Wakai, Toshifumi× Kamada, Tadashi× 崔 星× 鎌田 正 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To investigate whether a carbon ion beam has beneficial effects compared to X-ray by targeting putative cancer stem cells. Methods: Human liver cancer stem-like cells sorted from HepG2 and Huh7 cells were treated with or without carbon ion or X-ray irradiation and then colony formation assay, spheroid formation assay, gammaH2AX foci formation assays were performed. Histopathological changes of xenograft tumors after carbon or X-ray irradiation was also examined. Results: CD133+/CD90+ cells significantly have higher number of colony and spheroid compared to CD133-/CD90- cells. CD133+/CD90+ cells have shown more potential to form tumors in SCID mice than CD133-/CD90- cells. Colony assay showed that CD133+/CD90+ cells appeared to be radioresistant to both X-ray and carbon ion beam, but carbon beam was more effective at killing those of cancer stem-like cells. The number of gammaH2AX foci in CD133-/CD90- cells was higher than that of CD133+/CD90+ cells after irradiation with either X-ray or carbon ion beam. The number of gammaH2AX foci in CD133+/CD90+ cells was almost the same in the early time, but it persists for significantly longer in carbon ion beam irradiated cells compared to X-rays. Carbon ion irradiation with 15 Gy induced more severe xenograft tumor cell cavitation and fibrosis compared with 30 Gy X-rays, and tumors were significantly regressed following 30 Gy carbon ion irradiation. Conclusion: Taken together, carbon ion beam more effectively kills CD133+/CD90+ cancer stem-like cells via unrepairable DNA damage in vitro as well as tumor regression in vivo compared to X-ray. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 23rd Conference of the Asian Pacific Association for the Study of the Liver (APASL 2013), | |||||
| 発表年月日 | ||||||
| 日付 | 2013-06-10 | |||||
| 日付タイプ | Issued | |||||
