{"created":"2023-05-15T14:52:05.099653+00:00","id":71171,"links":{},"metadata":{"_buckets":{"deposit":"ef74abfd-e839-45da-bf51-9a9bfadb711e"},"_deposit":{"created_by":1,"id":"71171","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71171"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071171","sets":["10:28"]},"author_link":["699681","699684","699682","699685","699683"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-06-10","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: To investigate whether a carbon ion beam has beneficial effects compared to X-ray by targeting putative cancer stem cells.\nMethods: Human liver cancer stem-like cells sorted from HepG2 and Huh7 cells were treated with or without carbon ion or X-ray irradiation and then colony formation assay, spheroid formation assay, gammaH2AX foci formation assays were performed. Histopathological changes of xenograft tumors after carbon or X-ray irradiation was also examined.\nResults: CD133+/CD90+ cells significantly have higher number of colony and spheroid compared to CD133-/CD90- cells. CD133+/CD90+ cells have shown more potential to form tumors in SCID mice than CD133-/CD90- cells. Colony assay showed that CD133+/CD90+ cells appeared to be radioresistant to both X-ray and carbon ion beam, but carbon beam was more effective at killing those of cancer stem-like cells. The number of gammaH2AX foci in CD133-/CD90- cells was higher than that of CD133+/CD90+ cells after irradiation with either X-ray or carbon ion beam. The number of gammaH2AX foci in CD133+/CD90+ cells was almost the same in the early time, but it persists for significantly longer in carbon ion beam irradiated cells compared to X-rays. Carbon ion irradiation with 15 Gy induced more severe xenograft tumor cell cavitation and fibrosis compared with 30 Gy X-rays, and tumors were significantly regressed following 30 Gy carbon ion irradiation. \nConclusion: Taken together, carbon ion beam more effectively kills CD133+/CD90+ cancer stem-like cells via unrepairable DNA damage in vitro as well as tumor regression in vivo compared to X-ray.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"23rd Conference of the Asian Pacific Association for the Study of the Liver (APASL 2013),","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"699681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakai, Toshifumi"}],"nameIdentifiers":[{"nameIdentifier":"699682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"699683","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"699684","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"699685","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Distinct Effects of Carbon Ion Beam and X-ray on Putative Liver Cancer Stem Cell In Vitro and In Vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Distinct Effects of Carbon Ion Beam and X-ray on Putative Liver Cancer Stem Cell In Vitro and In Vivo"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-24"},"publish_date":"2013-06-24","publish_status":"0","recid":"71171","relation_version_is_last":true,"title":["Distinct Effects of Carbon Ion Beam and X-ray on Putative Liver Cancer Stem Cell In Vitro and In Vivo"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:54:22.702982+00:00"}