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The Role of XRCC4 in Human Colon Cancer Stem Cell Properties and Radiosensitivity
https://repo.qst.go.jp/records/71170
https://repo.qst.go.jp/records/711700e104acd-4314-400f-a63b-85c79ec03cf7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-06-24 | |||||
| タイトル | ||||||
| タイトル | The Role of XRCC4 in Human Colon Cancer Stem Cell Properties and Radiosensitivity | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Wakai, Toshifumi× Vares, Guillaume× Kamada, Tadashi× 崔 星× Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To investigate relationship between XRCC4 and cancer stem cell properties and further examine how XRCC4 involved in the radiosensitivity of putative human colon cancer stem cells after X-ray or carbon ion beam. Methods: Putative cancer stem cells sorted from HCT116-wild type (WT) and XRCC4 KO cells were treated with or without carbon ion or X-ray irradiation and then colony and spheroid formation assay, FACS analysis, gamma-H2AX foci assay, as well as in vivo tumor formation assay were performed. Results: FACS analysis showed that the percentage of CD44+ and ESA+ cells was significantly increased in XRCC4 KO cells (6.8%, and 7.2% vs 19.2% and 20%), whereas CD133+ was decreased (3.2% vs 1.6%) compared to HCT116-WT cells. The proportion of CD133+ and CD44+ cells was extremely increased in XRCC4 KO cells compared to HCT116-WT cells after X-ray irradiation. There was no change in proportion of ESA+ cells in HCT116-WT cells, but 10-fold enhancement of ESA+ cells was induced in HCT116-XRCC4 KO cells after X-ray irradiation. The number of colony and spheroid formed from CD133+, CD44+/ESA+ cells were significantly higher compared to that from CD133-, CD44-/ESA- cells in HCT116-XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. Analysis of cell survival fractions showed that CD133+, CD44+/ESA+ cells sorted from XRCC4 KO cells were predominantly radiosensitized compared to the that from HCT116-WT cells, especially after X-ray irradiation. A much more large number and large-sized gamma-H2AX foci were observed in CD44+/ESA+ cells sorted from XRCC4 KO cells compared to that from HCT116-WT cells, after 24 h carbon ion beam compared to X-ray irradiation. The in vivo tumorigenicity of XRCC4 KO cells is still retained and there are no differences between CD133+, CD44+/ESA+ cells and CD133-, CD44-/ESA- cells which sorted from XRCC4 KO cells. Conclusion: In conclusion, lack of XRCC4 significantly altered expression of cancer stem cell markers, radiosensitized cancer stem-like cells to both X-rays and carbon ion berams, suggesting that XRCC4 may play pivotal role in modulating cancer cell stemness. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Society for Stem Cell Research (ISSCR) 11 th Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2013-06-15 | |||||
| 日付タイプ | Issued | |||||
