{"created":"2023-05-15T14:52:05.032349+00:00","id":71170,"links":{},"metadata":{"_buckets":{"deposit":"7b18b341-73c5-4225-9da8-d0957cf971c8"},"_deposit":{"created_by":1,"id":"71170","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71170"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071170","sets":["10:28"]},"author_link":["699676","699677","699674","699679","699678","699675","699680"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-06-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: To investigate relationship between XRCC4 and cancer stem cell properties and further examine how XRCC4 involved in the radiosensitivity of putative human colon cancer stem cells after X-ray or carbon ion beam. \nMethods: Putative cancer stem cells sorted from HCT116-wild type (WT) and XRCC4 KO cells were treated with or without carbon ion or X-ray irradiation and then colony and spheroid formation assay, FACS analysis, gamma-H2AX foci assay, as well as in vivo tumor formation assay were performed.\nResults: FACS analysis showed that the percentage of CD44+ and ESA+ cells was significantly increased in XRCC4 KO cells (6.8%, and 7.2% vs 19.2% and 20%), whereas CD133+ was decreased (3.2% vs 1.6%) compared to HCT116-WT cells. The proportion of CD133+ and CD44+ cells was extremely increased in XRCC4 KO cells compared to HCT116-WT cells after X-ray irradiation. There was no change in proportion of ESA+ cells in HCT116-WT cells, but 10-fold enhancement of ESA+ cells was induced in HCT116-XRCC4 KO cells after X-ray irradiation. The number of colony and spheroid formed from CD133+, CD44+/ESA+ cells were significantly higher compared to that from CD133-, CD44-/ESA- cells in HCT116-XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. Analysis of cell survival fractions showed that CD133+, CD44+/ESA+ cells sorted from XRCC4 KO cells were predominantly radiosensitized compared to the that from HCT116-WT cells, especially after X-ray irradiation. A much more large number and large-sized gamma-H2AX foci were observed in CD44+/ESA+ cells sorted from XRCC4 KO cells compared to that from HCT116-WT cells, after 24 h carbon ion beam compared to X-ray irradiation. The in vivo tumorigenicity of XRCC4 KO cells is still retained and there are no differences between CD133+, CD44+/ESA+ cells and CD133-, CD44-/ESA- cells which sorted from XRCC4 KO cells. \nConclusion: In conclusion, lack of XRCC4 significantly altered expression of cancer stem cell markers, radiosensitized cancer stem-like cells to both X-rays and carbon ion berams, suggesting that XRCC4 may play pivotal role in modulating cancer cell stemness.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"International Society for Stem Cell Research (ISSCR) 11 th Annual Meeting","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"699674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakai, Toshifumi"}],"nameIdentifiers":[{"nameIdentifier":"699675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vares, Guillaume"}],"nameIdentifiers":[{"nameIdentifier":"699676","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"699677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"699678","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"699679","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"699680","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"The Role of XRCC4 in Human Colon Cancer Stem Cell Properties and Radiosensitivity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Role of XRCC4 in Human Colon Cancer Stem Cell Properties and Radiosensitivity"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-24"},"publish_date":"2013-06-24","publish_status":"0","recid":"71170","relation_version_is_last":true,"title":["The Role of XRCC4 in Human Colon Cancer Stem Cell Properties and Radiosensitivity"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:54:23.361739+00:00"}