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Exogenous carbon monoxide suppresses adaptive response induced in zebrafish embryos in vivo by microbeam protons
https://repo.qst.go.jp/records/71109
https://repo.qst.go.jp/records/71109566fb22a-2d55-4292-bc1c-74e3c6c4fc4c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-05-22 | |||||
| タイトル | ||||||
| タイトル | Exogenous carbon monoxide suppresses adaptive response induced in zebrafish embryos in vivo by microbeam protons | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Choi, V.W.Y.
× Choi, V.W.Y.× Y.P., Ng Candy× Kobayashi, Alisa× Konishi, Teruaki× Oikawa, Masakazu× H., Cheng Shuk× Yu, K.N.× 小林 亜利紗× 小西 輝昭× 及川 将一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Dechorionated embryos of the zebrafish, Danio rerio, irradiated at 5 h post fertilization (hpf) with 30 protons delivered to ten separate positions each with an energy of 3.4 MeV from the microbeam irradiation facility (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), developed radioadaptive response (RAR) against a subsequent challenging exposure of 2 Gy of X-ray irradiation at 10 hpf, evidenced by reduced apoptotic signals at 24 hpf revealed through terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay. The effects of the CO liberator tricarbonylchloro(glycinato)ruthenium (II) (CORM-3) on the induction of RAR were examined by transferring the irradiated embryos to freshly prepared medium with the chemical at different time points after the application of the priming dose. Our results showed that transfer of irradiated embryos into media with CORM-3 at 0, 1, 2 and 3 h after application of priming exposure significantly suppressed RAR, while transfer at 5 h did not suppress RAR. This was attributed to the protection of bystander cells from the released CO, which caused less de novo synthesis of factors and thus less efficient induction of RAR. Once the factors were synthesized, RAR was induced, which would not be further affected by the application of CORM-3 introduced at 5 h after the application of the priming dose. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Heavy Ion in Therapy and Space Radiation Spymposium 2013 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-18 | |||||
| 日付タイプ | Issued | |||||
