@misc{oai:repo.qst.go.jp:00071109,
 author = {Choi, V.W.Y. and Y.P., Ng Candy and Kobayashi, Alisa and Konishi, Teruaki and Oikawa, Masakazu and H., Cheng Shuk and Yu, K.N. and 小林 亜利紗 and 小西 輝昭 and 及川 将一},
 month = {May},
 note = {Dechorionated embryos of the zebrafish, Danio rerio, irradiated at 5 h post fertilization (hpf) with 30 protons delivered to ten separate positions each with an energy of 3.4 MeV from the microbeam irradiation facility (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), developed radioadaptive response (RAR) against a subsequent challenging exposure of 2 Gy of X-ray irradiation at 10 hpf, evidenced by reduced apoptotic signals at 24 hpf revealed through terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay. 
The effects of the CO liberator tricarbonylchloro(glycinato)ruthenium (II) (CORM-3) on the induction of RAR were examined by transferring the irradiated embryos to freshly prepared medium with the chemical at different time points after the application of the priming dose. Our results showed that transfer of irradiated embryos into media with CORM-3 at 0, 1, 2 and 3 h after application of priming exposure significantly suppressed RAR, while transfer at 5 h did not suppress RAR. This was attributed to the protection of bystander cells from the released CO, which caused less de novo synthesis of factors and thus less efficient induction of RAR. Once the factors were synthesized, RAR was induced, which would not be further affected by the application of CORM-3 introduced at 5 h after the application of the priming dose., Heavy Ion in Therapy and Space Radiation Spymposium 2013},
 title = {Exogenous carbon monoxide suppresses adaptive response induced in zebrafish embryos in vivo by microbeam protons},
 year = {2013}
}