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Visualisation of H2AX foci caused by heavy ion particle traversal; Distinction between core track versus non-track damage
https://repo.qst.go.jp/records/71104
https://repo.qst.go.jp/records/711046cf4a584-d437-4b8f-abe7-9a00d5edc336
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-05-21 | |||||
| タイトル | ||||||
| タイトル | Visualisation of H2AX foci caused by heavy ion particle traversal; Distinction between core track versus non-track damage | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakajima, Nakako
× Nakajima, Nakako× Hirayama, Ryoichi× Fujimori, Akira× Okayasu, Ryuichi× et.al× 中島 菜花子× 平山 亮一× 藤森 亮× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Heavy particle irradiation produces complex DNA double strand breaks (DSBs) which can arise from primary ionisation events within the particle trajectory. Additionally, secondary electrons, termed delta-electrons, which have a range of distributions can create low linear energy transfer (LET) damage within but also distant from the track.DNA damage by delta-electrons distant from the track has not previously been carefully characterised. Using imaging with deconvolution, we show that at 8 hours after exposure to Fe (~200 keV/micrometer) ions, gamma-H2AX foci forming at DSBs within the particle track are large and encompass multiple smaller and closely localised foci, which we designate as clustered gamma-H2AX foci. These foci are repaired with slow kinetics by DNA non-homologous end-joining (NHEJ) in G1 phase with the magnitude of complexity diminishing with time. These clustered foci (containing 10 or more individual foci) represent a signature of DSBs caused by high LET heavy particle radiation. We also identified simple gamma-H2AX foci distant from the track, which resemble those arising after X-ray exposure, which we attribute to low LET delta-electron induced DSBs. They are rapidly repaired by NHEJ. Clustered gamma-H2AX foci induced by heavy particle radiation cause prolonged checkpoint arrest compared to simple gamma-H2AX foci following X-irradiation. However, mitotic entry was observed when ~10 clustered foci remain. Thus, cells can progress into mitosis with multiple clusters of DSBs following the traversal of a heavy particle. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | HITSRS2013 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-18 | |||||
| 日付タイプ | Issued | |||||
