{"created":"2023-05-15T14:52:00.872758+00:00","id":71104,"links":{},"metadata":{"_buckets":{"deposit":"c4ad8a97-a3b1-4167-a82a-e419e79b92ca"},"_deposit":{"created_by":1,"id":"71104","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71104"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071104","sets":["10:28"]},"author_link":["698901","698900","698898","698895","698903","698902","698897","698896","698899"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-05-18","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Heavy particle irradiation produces complex DNA double strand breaks (DSBs) which can arise from primary ionisation events within the particle trajectory. Additionally, secondary electrons, termed delta-electrons, which have a range of distributions can create low linear energy transfer (LET) damage within but also distant from the track.DNA damage by delta-electrons distant from the track has not previously been carefully characterised. Using imaging with deconvolution, we show that at 8 hours after exposure to Fe (~200 keV/micrometer) ions, gamma-H2AX foci forming at DSBs within the particle track are large and encompass multiple smaller and closely localised foci, which we designate as clustered gamma-H2AX foci. These foci are repaired with slow kinetics by DNA non-homologous end-joining (NHEJ) in G1 phase with the magnitude of complexity diminishing with time. These clustered foci (containing 10 or more individual foci) represent a signature of DSBs caused by high LET heavy particle radiation. We also identified simple gamma-H2AX foci distant from the track, which resemble those arising after X-ray exposure, which we attribute to low LET delta-electron induced DSBs. They are rapidly repaired by NHEJ. Clustered gamma-H2AX foci induced by heavy particle radiation cause prolonged checkpoint arrest compared to simple gamma-H2AX foci following X-irradiation. However, mitotic entry was observed when ~10 clustered foci remain. Thus, cells can progress into mitosis with multiple clusters of DSBs following the traversal of a heavy particle.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"HITSRS2013","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakajima, Nakako"}],"nameIdentifiers":[{"nameIdentifier":"698895","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirayama, Ryoichi"}],"nameIdentifiers":[{"nameIdentifier":"698896","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"698897","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"698898","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"698899","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中島 菜花子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"698900","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平山 亮一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"698901","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"698902","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"698903","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Visualisation of H2AX foci caused by heavy ion particle traversal; Distinction between core track versus non-track damage","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Visualisation of H2AX foci caused by heavy ion particle traversal; Distinction between core track versus non-track damage"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-05-21"},"publish_date":"2013-05-21","publish_status":"0","recid":"71104","relation_version_is_last":true,"title":["Visualisation of H2AX foci caused by heavy ion particle traversal; Distinction between core track versus non-track damage"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:55:09.146806+00:00"}