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Enhanced cellular internalization of FGF1/CPPC fusion protein promoted its radioprotective effect in the hair follicles.
https://repo.qst.go.jp/records/71094
https://repo.qst.go.jp/records/71094e958f333-149c-4f32-ad25-3a93a8b110d5
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-05-14 | |||||
| タイトル | ||||||
| タイトル | Enhanced cellular internalization of FGF1/CPPC fusion protein promoted its radioprotective effect in the hair follicles. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakayama, Fumiaki
× Nakayama, Fumiaki× Umeda, Sachiko× Yasuda, Takeshi× Asada, Masahiro× Imamura, Toru× Imai, Takashi× 中山 文明× 梅田 禎子× 安田 武嗣× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Signals for FGF1 functions come from not only activated FGF receptors (FGFR) but also internalized FGF1. Although FGF11 subfamily members (FGF11-14) have structural similarity with FGF1, FGF11-14 possess unique C-terminal polypeptides. Novel cell-penetrating peptide domains (CPPC11-14) were identified from the C-terminal region of FGF11-14 proteins, which could readily deliver FGFs into cells independently of FGFRs. In this study, we created FGF1/CPPC fusion proteins (FGF1/CPPC11-14) following the alignment of FGF12 C-terminal region and evaluated the protective activity of FGF1/CPPC11-14 against radiation damage. FGF1/CPPC11-14 fusion proteins were internalized into the rat intestinal epithelial cell line IEC6 more efficiently than FGF1. However, the mitogenic activity of FGF1/CPPC12 through FGFR1c was less than that of FGF1. In contrast, FGF1/CPPC11-14 significantly reduced radiation-induced apoptosis in IEC6 cells in the presence of heparin, and the administration of FGF1/CPPC11-14 to BALB/c mice without heparin reduced the induction of apoptosis in hair bulbs 24 hafter irradiation. In addition, pre-treatment with FGF1/CPPC11-14 maintained K15 positive stem cells in the bulge after irradiation, although K15 positive stem cells decreased in anagen hair follicles after irradiation. These findings indicate that FGF1/CPPC fusion proteins protect the hair follicles against radiation-induced injury and suggest that cellular internalization of FGF1/CPPC11-14 is involved in their activities independently of FGFRs. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Investigative Dermatology EDINBURGH 2013 | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-11 | |||||
| 日付タイプ | Issued | |||||
