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SPIO-loaded unilamellar polyion complex vesicles (SPIO-Cy5-PICsomes) as a high relaxivity contrast agent for tumor
https://repo.qst.go.jp/records/71083
https://repo.qst.go.jp/records/71083c9622bef-7099-4306-8b70-c81a8b44bac1
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-04-30 | |||||
| タイトル | ||||||
| タイトル | SPIO-loaded unilamellar polyion complex vesicles (SPIO-Cy5-PICsomes) as a high relaxivity contrast agent for tumor | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kokuryo, Daisuke
× Kokuryo, Daisuke× Kano, Mitsunobu× Nishiyama, Nobuhiro× Saga, Tsuneo× Aoki, Ichio× et.al× 國領 大介× 狩野 光伸× 佐賀 恒夫× 青木 伊知男 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction The detection of early-stage tumors, especially in the metastatic case, is important for improving treatment efficacy and prolonging patient survival. Super paramagnetic iron-oxide (SPIO) nanoparticles are a highly sensitive MRI contrast agent and have the potential to be a powerful tool for a wide range of clinical and pre-clinical cancer studies.1 However, after intravenous administration conventional SPIO nanoparticles (eg ferucarbotran) in the bloodstream are rapidly captured by the reticuloendothelial system (RES), predominantly in the liver. Therefore, to avoid recognition by the RES and effectively target tumour tissue, it is necessary to equip the SPIO nanoparticles with a "stealth" capability. Previously, it has been reported that polyion complex vesicles (Nano-PICsomes), which are composed of biocompatible poly(ethylene glycol) (PEG) and poly(amino acid)s, can be easily engineered for size and are capable of prolonged circulation in the bloodstream.2, 3 In this paper, a novel MR and fluorescence contrast nanocarrier (named "SPIO-Cy5-PICsome"), that is specific for targeted tumor imaging and is based on the encapsulation of FDA-approved SPIO nanoparticles inside Nano-PICsomes, was synthesized and evaluated in vitro and in vivo for its ability to detect small tumors with high-field MRI. Materials and Methods The SPIO-Cy5-PICsomes were composed of ferucarbotran (Resovist®, Fujifilm RI Pharma) and two oppositely charged block copolymers: block-aniomer, fluorescence (Cy5)-labeled PEG-b-poly(α,β-aspartic acid) (Cy5-PEG-PAsp) and homo-catiomer, poly([5-aminopentyl]-α,β-aspartamide) (Homo-P(Asp-AP)) (Figure 1). A solution of Cy5-PEG-PAsp and ferucarbotran was prepared and then mixed with the Homo-P(Asp-AP) solution in an equal unit ratio of charged polymers, and stirred with a vortex mixer. The SPIO-Cy5-PICsome solution was then added to the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) solution for cross-linking. The size of the SPIO-Cy5-PICsomes was controlled to be around 100 nm. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ISMRM 21th Annual Meeting and Exhibition | |||||
| 発表年月日 | ||||||
| 日付 | 2013-04-26 | |||||
| 日付タイプ | Issued | |||||
