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The effects of X-irradiation on proliferation and differentiation of mouse ES-cell-derived neural stem cells.
https://repo.qst.go.jp/records/71003
https://repo.qst.go.jp/records/710032d8f0c4a-2334-48ec-8355-ff44cf448ddb
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-12-07 | |||||
| タイトル | ||||||
| タイトル | The effects of X-irradiation on proliferation and differentiation of mouse ES-cell-derived neural stem cells. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Isono, Mayu
× Isono, Mayu× Otsu, Masahiro× Konishi, Teruaki× Shiomi, Naoko× Nakayama, Takashi× Inoue, Nobuo× 小西 輝昭× 塩見 尚子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Normal brain formation requires regionally and temporally appropriate proliferation and differentiation of neural stem cells (NSCs) into neurons and glia. Exposure of the fetal brain to ionizing radiation cause congenital brain abnormalities, which is thought to result from defects in neurogenesis during the developing stage. Here, we investigated the effects of X-irradiation on proliferating homogenous NSCs, which were prepared from mouse embryonic stem cells by the Neural Stem Sphere method. Cells cultured in the proliferation medium, containing fibroblast growth factor-2 (FGF-2) were irradiated with X-rays and analyzed with growth curve measurements, flow cytometry, real-time RT-PCR, and immuno-fluorescence. The cells irradiated at a dose of > 5 Gy did not show an increase in number, but a gradual decrease was observed. The gradual decrease was explained by the loss of proliferation capability and also apoptosis. In contrast, the cells irradiated at a dose of 1 Gy stopped proliferation temporarily but recovered the proliferation capability. Moreover, sub-cultured at 96 hrs after irradiation, the cells proliferated logarithmically in the proliferation medium, and successfully differentiated into neurons and astrocytes in the differentiation medium. These results clearly demonstrate that exposure of the fetal brain to radiation at relatively high doses leads to congenital morphological and functional brain abnormalities. In addition, because brain development is a very stringently coordinated process from a clonological and regional viewpoint, these results suggest that the delay in cellular proliferation of NSCs after irradiation at relatively low doses may cause congenital brain abnormalities. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第2回国際放射線神経生物学会大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2011-12-03 | |||||
| 日付タイプ | Issued | |||||
