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The Role of XRCC4 in Sensitizing Human Colon Cancer Stem-Like Cells to X-ray or Carbon Ion Beam
https://repo.qst.go.jp/records/70969
https://repo.qst.go.jp/records/7096939291270-7d81-413f-8ab2-0acc504db964
Item type | 会議発表用資料 / Presentation(1) | |||||
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公開日 | 2012-11-07 | |||||
タイトル | ||||||
タイトル | The Role of XRCC4 in Sensitizing Human Colon Cancer Stem-Like Cells to X-ray or Carbon Ion Beam | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Sai, Sei
× Sai, Sei× Oonishi, Kazuhiko× Mori, Masahiko× Kamijo, Takehiko× Yamada, Shigeru× Kamada, Tadashi× 崔 星× 大西 和彦× 森 雅彦× 上條 岳彦× 山田 滋× 鎌田 正 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Inactivation of XRCC4 is associated with various human carcinogenesis. Here we report expression changes of cancer stem-like cell markers in XRCC4 deficient human colon cancer cells after X-ray or carbon ion beam. FACS analysis showed that the percentage of CD133+, CD44+ and ESA+ cells in HCT116-WT cells were 3.2%, 6.8%, and 7.2%, whereas 1.6%, 19.2% and 20% in HCT116-XRCC4 KO cells. The proportion of CD133+ and CD44+ cells was increased 3 to 4-fold after 2 Gy X-ray irradiation in HCT116-WT cells, in comparison, 4 to 6- fold increment of CD133+ and CD44+ cells was induced in HCT116-XRCC4 KO cells. There was no change in proportion of ESA+ cells in HCT116-WT cells, but 10-fold enhancement of ESA+ cells was induced in HCT116-XRCC4 KO cells after 2 Gy X-ray irradiation. Colony and spheroid formation from CD133+, CD44+/ESA+ cells were higher compared to CD133-, CD44-/ESA- cells in HCT116-XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. In conclusion, expression of cancer stem-like cell markers significantly increased after X-ray irradiation in XRCC4 deficient cells, suggesting that XRCC4 may be involved in cancer cell stemness. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 54th Annual Meeting of ASTRO | |||||
発表年月日 | ||||||
日付 | 2012-10-31 | |||||
日付タイプ | Issued |