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The Role of XRCC4 in Sensitizing Human Colon Cancer Stem-Like Cells to X-ray or Carbon Ion Beam
https://repo.qst.go.jp/records/70969
https://repo.qst.go.jp/records/7096939291270-7d81-413f-8ab2-0acc504db964
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-11-07 | |||||
| タイトル | ||||||
| タイトル | The Role of XRCC4 in Sensitizing Human Colon Cancer Stem-Like Cells to X-ray or Carbon Ion Beam | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Oonishi, Kazuhiko× Mori, Masahiko× Kamijo, Takehiko× Yamada, Shigeru× Kamada, Tadashi× 崔 星× 大西 和彦× 森 雅彦× 上條 岳彦× 山田 滋× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Inactivation of XRCC4 is associated with various human carcinogenesis. Here we report expression changes of cancer stem-like cell markers in XRCC4 deficient human colon cancer cells after X-ray or carbon ion beam. FACS analysis showed that the percentage of CD133+, CD44+ and ESA+ cells in HCT116-WT cells were 3.2%, 6.8%, and 7.2%, whereas 1.6%, 19.2% and 20% in HCT116-XRCC4 KO cells. The proportion of CD133+ and CD44+ cells was increased 3 to 4-fold after 2 Gy X-ray irradiation in HCT116-WT cells, in comparison, 4 to 6- fold increment of CD133+ and CD44+ cells was induced in HCT116-XRCC4 KO cells. There was no change in proportion of ESA+ cells in HCT116-WT cells, but 10-fold enhancement of ESA+ cells was induced in HCT116-XRCC4 KO cells after 2 Gy X-ray irradiation. Colony and spheroid formation from CD133+, CD44+/ESA+ cells were higher compared to CD133-, CD44-/ESA- cells in HCT116-XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. In conclusion, expression of cancer stem-like cell markers significantly increased after X-ray irradiation in XRCC4 deficient cells, suggesting that XRCC4 may be involved in cancer cell stemness. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 54th Annual Meeting of ASTRO | |||||
| 発表年月日 | ||||||
| 日付 | 2012-10-31 | |||||
| 日付タイプ | Issued | |||||
