{"created":"2023-05-15T14:51:54.977667+00:00","id":70969,"links":{},"metadata":{"_buckets":{"deposit":"82530ad2-a855-4a75-ad6b-fda8d7e286e2"},"_deposit":{"created_by":1,"id":"70969","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70969"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070969","sets":["10:28"]},"author_link":["697477","697478","697480","697476","697484","697473","697483","697482","697481","697479","697475","697474"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-10-31","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Inactivation of XRCC4 is associated with various human carcinogenesis. Here we report expression changes of cancer stem-like cell markers in XRCC4 deficient human colon cancer cells after X-ray or carbon ion beam. FACS analysis showed that the percentage of CD133+, CD44+ and ESA+ cells in HCT116-WT cells were 3.2%, 6.8%, and 7.2%, whereas 1.6%, 19.2% and 20% in HCT116-XRCC4 KO cells. The proportion of CD133+ and CD44+ cells was increased 3 to 4-fold after 2 Gy X-ray irradiation in HCT116-WT cells, in comparison, 4 to 6- fold increment of CD133+ and CD44+ cells was induced in HCT116-XRCC4 KO cells. There was no change in proportion of ESA+ cells in HCT116-WT cells, but 10-fold enhancement of ESA+ cells was induced in HCT116-XRCC4 KO cells after 2 Gy X-ray irradiation. Colony and spheroid formation from CD133+, CD44+/ESA+ cells were higher compared to CD133-, CD44-/ESA- cells in HCT116-XRCC4 KO cells, but extremely decreased compared to HCT116-WT cells. In conclusion, expression of cancer stem-like cell markers significantly increased after X-ray irradiation in XRCC4 deficient cells, suggesting that XRCC4 may be involved in cancer cell stemness.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"54th Annual Meeting of ASTRO","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"697473","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oonishi, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"697474","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"697475","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamijo, Takehiko"}],"nameIdentifiers":[{"nameIdentifier":"697476","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"697477","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"697478","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"697479","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 和彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"697480","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森 雅彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"697481","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上條 岳彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"697482","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"697483","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"697484","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"The Role of XRCC4 in Sensitizing Human Colon Cancer Stem-Like Cells to X-ray or Carbon Ion Beam","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Role of XRCC4 in Sensitizing Human Colon Cancer Stem-Like Cells to X-ray or Carbon Ion Beam"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-07"},"publish_date":"2012-11-07","publish_status":"0","recid":"70969","relation_version_is_last":true,"title":["The Role of XRCC4 in Sensitizing Human Colon Cancer Stem-Like Cells to X-ray or Carbon Ion Beam"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:56:43.287139+00:00"}