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Loss of the BRCA1-interacting helicase BRIP1 results in abnormal mammary acinar morphogenesis
https://repo.qst.go.jp/records/70835
https://repo.qst.go.jp/records/70835c30c4850-95a2-448d-88e3-eee1c082c229
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-07-24 | |||||
| タイトル | ||||||
| タイトル | Loss of the BRCA1-interacting helicase BRIP1 results in abnormal mammary acinar morphogenesis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Daino, Kazuhiro
× Daino, Kazuhiro× Imaoka, Tatsuhiko× Morioka, Takamitsu× Nishimura, Mayumi× Shimada, Yoshiya× 臺野 和広× 今岡 達彦× 森岡 孝満× 西村 まゆみ× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | BRIP1 is a DNA helicase that directly interacts with the C-terminal BRCT repeat of the breast cancer susceptibility protein BRCA1 and plays an important role in BRCA1-dependent DNA repair and DNA damage-induced checkpoint control. Recent studies implicate BRIP1 as a moderate/low-penetrance breast cancer susceptibility gene. However, the phenotypic effects of BRIP1 dysfunction and its role in breast cancer tumorigenesis remain unclear. In this study, we used the three-dimensional culture of human mammary epithelial cells as a model of mammary gland morphogenesis, to explore the function of BRIP1 in acinar morphogenesis of mammary epithelial cells. BRIP1 knockdown in non-malignant MCF-10A mammary epithelial cells by RNA interference induced neoplastic-like changes such as abnormal cell adhesion, increased cell proliferation, large and irregular-shaped acini, invasive growth, and defective lumen formation. BRIP1-knockdown cells showed dysregulation of multiple signaling pathways and tumor-associated genes including SATB1, a key transcriptional regulator that promotes tumor growth and metastasis of breast cancer. These results suggest that BRIP1, by regulating multiple tumor-associated genes and signaling pathways, plays important roles not only in the development of mammary glands but also in the neoplastic conversion of mammary epithelial cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 22nd Biennial Congress of the European Association for Cancer Research | |||||
| 発表年月日 | ||||||
| 日付 | 2012-07-10 | |||||
| 日付タイプ | Issued | |||||
