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3'-Phosphoadenosine 5'-Phosphosulfate Transporter (PAPST) Enhanced Sulfation of Keratan Sulfate Proteoglycan to Reduce Radiation-Induced Apoptosis in a Human Burkitt Lymphoma Cell Line
https://repo.qst.go.jp/records/70737
https://repo.qst.go.jp/records/70737e64d9c1e-df5a-4a53-8886-5fb1ca0d055d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-05-11 | |||||
| タイトル | ||||||
| タイトル | 3'-Phosphoadenosine 5'-Phosphosulfate Transporter (PAPST) Enhanced Sulfation of Keratan Sulfate Proteoglycan to Reduce Radiation-Induced Apoptosis in a Human Burkitt Lymphoma Cell Line | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakayama, Fumiaki
× Nakayama, Fumiaki× Umeda, Sachiko× Ichimiya, Tomomi× Kamiyama, Shin× Nishihara, Shoko× Imai, Takashi× 中山 文明× 梅田 禎子× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | [Objectives] Sulfation of various molecules plays an essential role in biological and pathological processes. Two members of 3'-Phosphoadenosine 5'-phosphosulfate transporters (PAPSTs), PAPST1 and PAPAT2, are currently identified in humans. However, the influence of sulfate transporter on radiosensitvity of malignant tumors remains unknown. This study aimed at clarifying the role of sulfate transporter in radiation-induced apoptosis of lymphoma cells. [Methods] A human Burkitt' s lymphoma cell line, Namalwa, was transfected with an expression vector of human PAPST1 or PAPST2, or siRNA targeted against each PAPST. Apoptosis was evaluated by Hoechst 33258 staining 24 h after irradiation with X-rays or C-ion. [Results] The level of PAPST1 transcripts was approximately 5-fold higher than that of PAPST2 in Namalwa cells. Overexpression of each PAPST reduced radiation-induced apoptosis, whereas the repression of PAPST expression enhanced apoptosis. In contrast, keratan sulafate proteoglycan (KSPG) was expressed on the cell surface of Namalwa cells, and depletion of KS with keratanase significantly increased radiation-induced apoptosis. Three (CHST2, 6, and 7) of five sulfotransferases involved in KS synthesis were expressed in Namalwa cells and the sulfation catalyzed by all three sulfotransferases promoted anti-apoptotic effects of KSPG. PAPST1 enhanced phosphorylation of p38 MAPK and Akt in Namalwa cells, whereas inhibition of p38 MAPK or PI-3K increased radiation-induced apoptosis. [Conclussions] PAPST inhibited radiation-induced apoptosis through sulfation of KSPG. These findings suggest that KSPG plays an important role in anti-apoptotic signaling in human Burkitt' s lymphoma, and PAPST is useful to increase the efficacy of radiotherapy and decrease side effects through the regulation of KSPG. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 2nd Japan-China Symposium on Cancer Research | |||||
| 発表年月日 | ||||||
| 日付 | 2012-05-11 | |||||
| 日付タイプ | Issued | |||||
