{"created":"2023-05-15T14:51:44.796693+00:00","id":70737,"links":{},"metadata":{"_buckets":{"deposit":"82f0a171-8976-4faf-8a50-b761a10bf090"},"_deposit":{"created_by":1,"id":"70737","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70737"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070737","sets":["10:28"]},"author_link":["694886","694887","694888","694889","694884","694891","694890","694885","694883"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-05-11","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[Objectives] Sulfation of various molecules plays an essential role in biological and pathological processes.\nTwo members of 3'-Phosphoadenosine 5'-phosphosulfate transporters (PAPSTs), PAPST1 and PAPAT2, are\ncurrently identified in humans. However, the influence of sulfate transporter on radiosensitvity of malignant\ntumors remains unknown. This study aimed at clarifying the role of sulfate transporter in radiation-induced\napoptosis of lymphoma cells.\n[Methods] A human Burkitt' s lymphoma cell line, Namalwa, was transfected with an expression vector of\nhuman PAPST1 or PAPST2, or siRNA targeted against each PAPST. Apoptosis was evaluated by Hoechst 33258\nstaining 24 h after irradiation with X-rays or C-ion.\n[Results] The level of PAPST1 transcripts was approximately 5-fold higher than that of PAPST2 in Namalwa\ncells. Overexpression of each PAPST reduced radiation-induced apoptosis, whereas the repression of PAPST\nexpression enhanced apoptosis. In contrast, keratan sulafate proteoglycan (KSPG) was expressed on the cell\nsurface of Namalwa cells, and depletion of KS with keratanase significantly increased radiation-induced\napoptosis. Three (CHST2, 6, and 7) of five sulfotransferases involved in KS synthesis were expressed in\nNamalwa cells and the sulfation catalyzed by all three sulfotransferases promoted anti-apoptotic effects of\nKSPG. PAPST1 enhanced phosphorylation of p38 MAPK and Akt in Namalwa cells, whereas inhibition of p38\nMAPK or PI-3K increased radiation-induced apoptosis.\n[Conclussions] PAPST inhibited radiation-induced apoptosis through sulfation of KSPG. These findings\nsuggest that KSPG plays an important role in anti-apoptotic signaling in human Burkitt' s lymphoma, and PAPST\nis useful to increase the efficacy of radiotherapy and decrease side effects through the regulation of KSPG.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 2nd Japan-China Symposium on Cancer Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakayama, Fumiaki"}],"nameIdentifiers":[{"nameIdentifier":"694883","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Umeda, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"694884","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichimiya, Tomomi"}],"nameIdentifiers":[{"nameIdentifier":"694885","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamiyama, Shin"}],"nameIdentifiers":[{"nameIdentifier":"694886","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishihara, Shoko"}],"nameIdentifiers":[{"nameIdentifier":"694887","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"694888","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中山 文明","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"694889","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"梅田 禎子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"694890","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"694891","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"3'-Phosphoadenosine 5'-Phosphosulfate Transporter (PAPST) Enhanced Sulfation of Keratan Sulfate Proteoglycan to Reduce Radiation-Induced Apoptosis in a Human Burkitt Lymphoma Cell Line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"3'-Phosphoadenosine 5'-Phosphosulfate Transporter (PAPST) Enhanced Sulfation of Keratan Sulfate Proteoglycan to Reduce Radiation-Induced Apoptosis in a Human Burkitt Lymphoma Cell Line"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-11"},"publish_date":"2012-05-11","publish_status":"0","recid":"70737","relation_version_is_last":true,"title":["3'-Phosphoadenosine 5'-Phosphosulfate Transporter (PAPST) Enhanced Sulfation of Keratan Sulfate Proteoglycan to Reduce Radiation-Induced Apoptosis in a Human Burkitt Lymphoma Cell Line"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:59:26.800426+00:00"}