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Tumor-specific imaging with radiolabeled human anti-transferrin receptor antibody in pancreatic cancer mouse model
https://repo.qst.go.jp/records/70510
https://repo.qst.go.jp/records/705105b6fee8c-1adb-4da0-9ec9-6e1d6a44eb05
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-09-06 | |||||
| タイトル | ||||||
| タイトル | Tumor-specific imaging with radiolabeled human anti-transferrin receptor antibody in pancreatic cancer mouse model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sugyou, Aya
× Sugyou, Aya× Tsuji, Atsushi× Sudou, Hitomi× Yoshida, Chisato× Kurosawa, Yoshikazu× Saga, Tsuneo× et.al× 須尭 綾× 辻 厚至× 須藤 仁美× 吉田 千里× 黒澤 良和× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We radiolabeled the antibody with 125I by the chloramine-T method, and with 67Ga or 89Zr using deferoxamine. We conducted in vitro cell binding and competitive inhibition assays using a human pancreatic carcinoma cell line, MIAPaCa-2, which highly expressed TfR and a mouse fibroblast cell line A4, which highly expressed human HER2 but not human TfR. We inoculated MIAPaCa-2 and A4 cells subcutaneously into each side of hind limb and conducted PET imaging from one to six days postinjection of the 89Zr-labeled antibody. The cell binding assay showed that radiolabeled antibodies specifically bound to MIAPaCa-2 cells, but not to A4 cells. The Bmax of 125I-, 67Ga- and 89Zr-labeled antibodies was 69, 86 and 64 %, respectively. The competitive inhibition assay showed that Kd of 125I-labeled antibodies was 2 nM, respectively. Temporal PET imaging with the 89Zr -labeled antibody showed that the MIAPaca-2 tumor was readily visualized on day 1 postinjection and became clearer thereafter, and the uptake in the MIAPaca-2 tumor increased with time and reached 24.8% of injected dose per gram on day 6, whereas that in the A4 tumor was low and decreased with time. In conclusion, the radiolabeled anti-TfR antibody could be applicable for TfR-specific PET imaging and help in selecting appropriate patients for TfR-targeted treatments. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 14th International Congress of Radiation Research(ICRR’2011) | |||||
| 発表年月日 | ||||||
| 日付 | 2011-09-01 | |||||
| 日付タイプ | Issued | |||||
