{"created":"2023-05-15T14:51:34.879876+00:00","id":70510,"links":{},"metadata":{"_buckets":{"deposit":"4b2dfe25-1ab9-44e5-835e-57f7d4c328e3"},"_deposit":{"created_by":1,"id":"70510","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70510"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070510","sets":["10:28"]},"author_link":["692421","692420","692428","692417","692418","692423","692422","692426","692419","692427","692429","692424","692425"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-09-01","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We radiolabeled the antibody with 125I by the chloramine-T method, and with 67Ga or 89Zr using deferoxamine. We conducted in vitro cell binding and competitive inhibition assays using a human pancreatic carcinoma cell line, MIAPaCa-2, which highly expressed TfR and a mouse fibroblast cell line A4, which highly expressed human HER2 but not human TfR. We inoculated MIAPaCa-2 and A4 cells subcutaneously into each side of hind limb and conducted PET imaging from one to six days postinjection of the 89Zr-labeled antibody. The cell binding assay showed that radiolabeled antibodies specifically bound to MIAPaCa-2 cells, but not to A4 cells. The Bmax of 125I-, 67Ga- and 89Zr-labeled antibodies was 69, 86 and 64 %, respectively. The competitive inhibition assay showed that Kd of 125I-labeled antibodies was 2 nM, respectively. Temporal PET imaging with the 89Zr -labeled antibody showed that the MIAPaca-2 tumor was readily visualized on day 1 postinjection and became clearer thereafter, and the uptake in the MIAPaca-2 tumor increased with time and reached 24.8% of injected dose per gram on day 6, whereas that in the A4 tumor was low and decreased with time. In conclusion, the radiolabeled anti-TfR antibody could be applicable for TfR-specific PET imaging and help in selecting appropriate patients for TfR-targeted treatments.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"14th International Congress of Radiation Research(ICRR’2011)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sugyou, Aya"}],"nameIdentifiers":[{"nameIdentifier":"692417","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"692418","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sudou, Hitomi"}],"nameIdentifiers":[{"nameIdentifier":"692419","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, Chisato"}],"nameIdentifiers":[{"nameIdentifier":"692420","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kurosawa, Yoshikazu"}],"nameIdentifiers":[{"nameIdentifier":"692421","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"692422","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"692423","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須尭 綾","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692424","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692425","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須藤 仁美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692426","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉田 千里","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692427","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"黒澤 良和","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692428","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692429","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Tumor-specific imaging with radiolabeled human anti-transferrin receptor antibody in pancreatic cancer mouse model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tumor-specific imaging with radiolabeled human anti-transferrin receptor antibody in pancreatic cancer mouse model"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-09-06"},"publish_date":"2011-09-06","publish_status":"0","recid":"70510","relation_version_is_last":true,"title":["Tumor-specific imaging with radiolabeled human anti-transferrin receptor antibody in pancreatic cancer mouse model"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:02:06.146071+00:00"}