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Current Status of Microbeam Irradiation System, SPICE-NIRS
https://repo.qst.go.jp/records/70492
https://repo.qst.go.jp/records/704924e1ee8d5-c717-4839-8ee5-e238a7ac84de
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-09-05 | |||||
| タイトル | ||||||
| タイトル | Current Status of Microbeam Irradiation System, SPICE-NIRS | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Konishi, Teruaki
× Konishi, Teruaki× Oikawa, Masakazu× Ishikawa, Takahiro× Isono, Mayu× Shiomi, Naoko× Maeda, Takeshi× Suya, Noriyoshi× 小西 輝昭× 及川 将一× 石川 剛弘× 塩見 尚子× 前田 武× 酢屋 徳啓 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Single-cell microbeam irradiation systems have become significant tools in the field of radiation biology and have discovered much important evidence that have never been described in the study using conventional broad beam irradiation. Single Particle Irradiation System to Cell, SPICE at National Institute of Radiological Sciences (NIRS) is a proton microbeam irradiation system developed for low dose radiation effect studies, such as for the cellular response of targeted and non-targeted effects. SPICE provides 3.4 MeV proton microbeam by using two slit system and a mono-bloc triplet Q lens so as to exclude such low-energy particle components by scattering seen with the collimation method. Approximately, 2 um in a diameter beam are routinely available. A cell dish is placed on the voice coil stage of the microscope system, which also contains a fluorescent microscope and a CCD camera. As a routine procedure, cell nuclei are dyed with 1 uM Hoechst 33342, and the X-Y coordinates of the cell position in the dish are calculated automatically corresponding to the obtained fluorescent images. Each nucleus can be irradiated with the pre-set number of protons with a probability of 96.6 % accuracy, and will be irradiated according to the calculated coordinates with a maximum speed of 400 cells per minutes by controlling the voice coil motor stage. Approximately, two thousand cells in 25 mm2 area per dish can be irradiated within 10 minutes including image capturing, cell recognition, and irradiation. In this presentation, collaborative researches such as targeted and non-targeted effects in mammalian cells and also in vivo studies performed using SPICE will be introduced. Electrostatic accelerator facility of NIRS received a severe damage from the Tohoku-Kanto earthquake on March 11th, 2011, and currently, SPICE is under construction. Hopefully, SPICE will be renewed, and resume its operation by the beginning of April of 2012. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 14th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2011-09-01 | |||||
| 日付タイプ | Issued | |||||
