@misc{oai:repo.qst.go.jp:00070492,
 author = {Konishi, Teruaki and Oikawa, Masakazu and Ishikawa, Takahiro and Isono, Mayu and Shiomi, Naoko and Maeda, Takeshi and Suya, Noriyoshi and 小西 輝昭 and 及川 将一 and 石川 剛弘 and 塩見 尚子 and 前田 武 and 酢屋 徳啓},
 month = {Sep},
 note = {Single-cell microbeam irradiation systems have become significant tools in the field of radiation biology and have discovered much important evidence that have never been described in the study using conventional broad beam irradiation. Single Particle Irradiation System to Cell, SPICE at National Institute of Radiological Sciences (NIRS) is a proton microbeam irradiation system developed for low dose radiation effect studies, such as for the cellular response of targeted and non-targeted effects. SPICE provides 3.4 MeV proton microbeam by using two slit system and a mono-bloc triplet Q lens so as to exclude such low-energy particle components by scattering seen with the collimation method. Approximately, 2 um in a diameter beam are routinely available. A cell dish is placed on the voice coil stage of the microscope system, which also contains a fluorescent microscope and a CCD camera. As a routine procedure, cell nuclei are dyed with 1 uM Hoechst 33342, and the X-Y coordinates of the cell position in the dish are calculated automatically corresponding to the obtained fluorescent images. Each nucleus can be irradiated with the pre-set number of protons with a probability of 96.6 % accuracy, and will be irradiated according to the calculated coordinates with a maximum speed of 400 cells per minutes by controlling the voice coil motor stage. Approximately, two thousand cells in 25 mm2 area per dish can be irradiated within 10 minutes including image capturing, cell recognition, and irradiation. In this presentation, collaborative researches such as targeted and non-targeted effects in mammalian cells and also in vivo studies performed using SPICE will be introduced.
  Electrostatic accelerator facility of NIRS received a severe damage from the Tohoku-Kanto earthquake on March 11th, 2011, and currently, SPICE is under construction. Hopefully, SPICE will be renewed, and resume its operation by the beginning of April of 2012., 14th International Congress of Radiation Research},
 title = {Current Status of Microbeam Irradiation System, SPICE-NIRS},
 year = {2011}
}