WEKO3
アイテム
第32回日本分子生物学会年会
https://repo.qst.go.jp/records/70158
https://repo.qst.go.jp/records/70158fc540807-96c4-4fb2-a399-e301f1953555
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-06-16 | |||||
| タイトル | ||||||
| タイトル | 第32回日本分子生物学会年会 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
安田, 武嗣
× 安田, 武嗣× 安田 武嗣 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | PIDD (p53-induced protein with a death domain) plays a critical role in the activation of caspase-2 to trigger apoptosis induced by DNA damage through the formation of a so-called PIDDosome, which contains the adaptor protein RAIDD and caspase-2. We found that transcription of PIDD was induced after exposure to ionizing radiation in rat small intestinal epithelial cell line (IEC6) cells. Yeast two-hybrid analysis indicated that the death domain of rat PIDD interacts with RAIDD. Interestingly, a rat C-terminal PIDD fragment (residues 773-917) containing the death domain interacts with RAIDD much more tightly than the longer PIDD fragment (residues 610-917). When the PIDD (773-917) fragment was overexpressed in theses cells, the PIDD-mediated activation of caspase-2 was dominant-negatively inhibited. In order to use the PIDD (773-917) fragment as an anti-apoptotic drug, we purified a recombinant PIDD (773-917) fragment fused with a basic 11-amino acid peptide derived from the HIV-Tat protein which facilitates uptake of the protein into mammalian cells with high efficiency. When PIDD (773-917)-TAT was added to the IEC6 cells, the protein was efficiently delivered into the cells within an hour. Furthermore, we observed that ionizing radiation-induced activation of caspase-2 and caspase-9 was inhibited when PIDD (773-917)-TAT was added to the IEC6 cells. These results suggest that PIDD (773-917)-TAT could protect gastrointestinal cells from ionizing radiation-induced cell death. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第32回日本分子生物学会年会 | |||||
| 発表年月日 | ||||||
| 日付 | 2009-12-12 | |||||
| 日付タイプ | Issued | |||||
