{"created":"2023-05-15T14:51:19.237379+00:00","id":70158,"links":{},"metadata":{"_buckets":{"deposit":"156e75f9-12f5-42d6-9307-0ca4e260c753"},"_deposit":{"created_by":1,"id":"70158","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70158"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070158","sets":["10:28"]},"author_link":["688894","688893"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-12-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"PIDD (p53-induced protein with a death domain) plays a critical role in the activation of caspase-2 to trigger apoptosis induced by DNA damage through the formation of a so-called PIDDosome, which contains the adaptor protein RAIDD and caspase-2.     We found that transcription of PIDD was induced after exposure to ionizing radiation in rat small intestinal epithelial cell line (IEC6) cells.  Yeast two-hybrid analysis indicated that the death domain of rat PIDD interacts with RAIDD.  Interestingly, a rat C-terminal PIDD fragment (residues 773-917) containing the death domain interacts with RAIDD much more tightly than the longer PIDD fragment (residues 610-917).  When the PIDD (773-917) fragment was overexpressed in theses cells, the PIDD-mediated activation of caspase-2 was dominant-negatively inhibited.  In order to use the PIDD (773-917) fragment as an anti-apoptotic drug, we purified a recombinant PIDD (773-917) fragment fused with a basic 11-amino acid peptide derived from the HIV-Tat protein which facilitates uptake of the protein into mammalian cells with high efficiency.  When PIDD (773-917)-TAT was added to the IEC6 cells, the protein was efficiently delivered into the cells within an hour.  Furthermore, we observed that ionizing radiation-induced activation of caspase-2 and caspase-9 was inhibited when PIDD (773-917)-TAT was added to the IEC6 cells.  These results suggest that PIDD (773-917)-TAT could protect gastrointestinal cells from ionizing radiation-induced cell death.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第32回日本分子生物学会年会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安田, 武嗣"}],"nameIdentifiers":[{"nameIdentifier":"688893","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安田 武嗣","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688894","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"第32回日本分子生物学会年会","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"第32回日本分子生物学会年会"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-16"},"publish_date":"2010-06-16","publish_status":"0","recid":"70158","relation_version_is_last":true,"title":["第32回日本分子生物学会年会"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:06:11.306463+00:00"}