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Bacteria contributing to the behavior of radiocarbon in sodium acetate
https://repo.qst.go.jp/records/70127
https://repo.qst.go.jp/records/70127878fb2dc-933d-4519-8f89-d18e8e847092
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-05-31 | |||||
| タイトル | ||||||
| タイトル | Bacteria contributing to the behavior of radiocarbon in sodium acetate | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishii, Nobuyoshi
× Ishii, Nobuyoshi× Uchida, Shigeo× 石井 伸昌× 内田 滋夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the safety assessment for geological disposal of transuranic (TRU) waste, better understanding of the behaviors of the contaminating radionuclides in agricultural soils are required because there are concerns about their migration of radionuclides from the geological disposal site to the living sphere through groundwater. Radiocarbon (14C) is one of the dominant radionuclides contributing to the dose from TRU waste. Recently, it was found that 14C atoms in organic matter such as carboxylic acids are released from metallic TRU waste. In our previous study using [1,2-14C] sodium acetate, we found that bacteria are responsible for the behavior of 14C atoms in organic matter in Japanese paddy soils. In addition, bacteria that existed in deionized water used for flooding of agricultural soils in our batch culture experiments were found to utilized sodium acetate. The objectives of the present study are to isolate the acetate-utilizing bacterium and to identify the species name. R2A agar plates containing [1,2-14C] sodium acetate were used to isolate the acetate-utilizing bacterium. Autoradiography images showed that all colonies on the R2A plates were positive for 14C utilization. Then we isolated one of the colonies. A partial sequence of 16S rRNA gene was used for the identification of the bacterium. Results of the sequence indicated that the bacterium had high homology (99.4%) to Burkholderia gladioli. B. gladioli is found in soil, water, and the rhizosphere. Bacteria that affect the behavior of 14C atoms in organic matter will be omnipresent in agricultural soils. Relationships between bacteria and the behavior of 14C atoms must be paid attention to in the safety assessment for geological disposal of TRU waste. This work has been partially supported by the Agency for Natural Resources and Energy, the Ministry of Economy, Trade and Industry (METI), Japan. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The Third Asian and Oceanic Congress on Radiation Protection (AOCRP-3) | |||||
| 発表年月日 | ||||||
| 日付 | 2010-05-27 | |||||
| 日付タイプ | Issued | |||||
