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ABROGATION OF THE G2/M ARREST ENHANCED THE CYTOTOXIC EFFECTS BY CARBON-ION BEAMS
https://repo.qst.go.jp/records/70036
https://repo.qst.go.jp/records/700366f8c22f1-2256-4136-a25e-13d02f0912a7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-02-19 | |||||
| タイトル | ||||||
| タイトル | ABROGATION OF THE G2/M ARREST ENHANCED THE CYTOTOXIC EFFECTS BY CARBON-ION BEAMS | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matsumoto, Yoshitaka
× Matsumoto, Yoshitaka× Hirayama, Ryoichi× Sai, Sei× Ando, Koichi× Uzawa, Akiko× Takase, Nobuhiro× Koike, Sachiko× Okayasu, Ryuichi× Furusawa, Yoshiya× 松本 孔貴× 平山 亮一× 崔 星× 鵜澤 玲子× 高瀬 信宏× 小池 幸子× 岡安 隆一× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of this study is to clarify cell cycle distribution and cytotoxicity after carbon-ion beams (C-ions). One human malignant melanoma cell line, HMV-I was exposed to X-rays or 290 MeV/u C-ions at the center of SOBP. We applied flow cytometry technique and colony formation assay. C-ions induced G2/M arrest effectively more than X-rays at 24 h. The iso-effect dose, i.e. the 10% survival dose (5.0Gy for X-rays, 2.7Gy for C-ions) was used to examine the cell cycle kinetics. G2/M fraction increased immediately after irradiations, and showed a peak at 15 h. G2/M arrest cells were stayed long time after C-ions, whereas it released quickly after X-rays. In addition, the mRNA expression of molecules related with the arrest was suppressed with D10 dose and the effects were transitory after X-rays, whereas C-ions showed prolonged suppression. The arrest was released significantly by caffeine, and the cell killing by C-ions was enhanced with caffeine together. It is suggested that the release from G2/M arrest after C-ions can enhance the cytotoxic effects. Repair of complex DNA damage by C-ions takes long time. When it released intermediate of the process, incomplete repaired damage cause cell death. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | MICROS 2009 15th International Symposium on Microdosimetry | |||||
| 発表年月日 | ||||||
| 日付 | 2009-10-30 | |||||
| 日付タイプ | Issued | |||||
