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Dependence in bystander cellular responses in mutation induction on normal human fibroblasts induced by the pre-treatment with different radiation types
https://repo.qst.go.jp/records/69752
https://repo.qst.go.jp/records/6975255e083e3-0bbc-473b-807b-240079a0f1bf
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-05-22 | |||||
| タイトル | ||||||
| タイトル | Dependence in bystander cellular responses in mutation induction on normal human fibroblasts induced by the pre-treatment with different radiation types | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suzuki, Masao
× Suzuki, Masao× Tsuruoka, Chizuru× Kitamura, Hisashi× Hua, Liu Cui× Uchihori, Yukio× 鈴木 雅雄× 鶴岡 千鶴× 北村 尚× 劉 翠華× 内堀 幸夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To investigate cellular responses in normal human fibroblasts induced with low dose irradiations of qualitatively different radiation types. Methods: Normal human fibroblasts were pre-treated with low-fluence irradiations (~1mGy/7-8h) of 137Cs gamma rays, 241Am-Be neutrons (maximum energy ; 11.5MeV, average energy ; 5.0MeV), helium (LET=2.3keV/µm), carbon (LET=13.3keV/µm) and iron ions (LET=200keV/µm) before applying irradiation with a 200kV X-ray challenging dose (1.5Gy). The 3 different ion beams were produced by the Heavy Ion Medical Accelerator in Chiba (HIMAC) at National Institute of Radiological Sciences. Results: There was no difference in X-ray-induced mutation frequency, which was detected with hprt locus detected as 6-thioguanine resistant clones, between un-pretreated and gamma-ray pre-treated cells. In the case of the pre-treatment with high-LET ions, mutation frequency was around 4.0 times higher in carbon- and 1.9 times higher in helium-ion pre-treated cells than in un-pretreated cells, while no difference was observed in iron-ion pre-treated cells. On the contrary, mutation frequency in cells pre-treated with neutrons was reduced at 15% of un-pretreated cells. To identify possible mechanism(s) of the observed cellular response, cells were treated with a specific inhibitor of gap-junction mediated cell-cell communication. The results clearly showed that the observed enhancement of X-ray-induced mutation frequency in cells pre-treated with helium and carbon ions was reduced at the control level and reduction in cells pre-treated with neutrons was came back to the control level. Conclusion: There is evidence that gap-junction mediated cell-cell communication play an important role of inducing different cellular responses by pre-treatment with different radiation types. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第二回アジア放射線連合会議（2nd Asian Congress of Radiation Research） | |||||
| 発表年月日 | ||||||
| 日付 | 2009-05-20 | |||||
| 日付タイプ | Issued | |||||
