WEKO3
アイテム
Neurovascular coupling studies in awake-behaving mice.
https://repo.qst.go.jp/records/69726
https://repo.qst.go.jp/records/697262703e64c-4648-421a-8782-557221bcf14f
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-03-30 | |||||
| タイトル | ||||||
| タイトル | Neurovascular coupling studies in awake-behaving mice. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takuwa, Hiroyuki
× Takuwa, Hiroyuki× Masamoto, Kazuto× Obata, Takayuki× Kanno, Iwao× 田桑 弘之× 正本 和人× 小畠 隆行× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The mechanism regulating cerebral blood flow (CBF) during brain functions (neurovascular coupling) was widely investigated with animals under the anesthetized condition. However, anesthesia is known to greatly affect neurovascular coupling and systemic physiology. Therefore, the present study aimed to develop a novel model for neurovascular coupling studies in awake-behaving mice. The male C57BL/6J mice (5-7 weeks) were initially anesthetized with isoflurane (2.5%) for the preparation of head attachment to apparatus. The skin of the head was cut and a metal head plate was attached to the skull. The animal was tethered by screwing the head plate onto apparatus, and then the anesthesia was discontinued for recovery of the animal. The regional CBF in the somatosensory barrel cortex was continuously measured with laser-Doppler flowmetry (LDF). Behavior of animal was also recorded using a digital camera. Whisker stimulation (frequency 10 Hz and duration 10 sec) was induced to the contralateral side of LDF recording site. During experiments, the animals showed no signs of struggling against the head restraint. Based on the type of the animal behavior, the experiment period was separated into three phases (grooming, running and resting). The CBF was observed to be stable during both resting and running phases, showing negligible motion artifacts. The increase in CBF was observed during grooming and whisker stimulation, which was higher than that in the anesthetized condition. In conclusion, the present model allows for the dynamic study of neurovascular coupling in awake-behaving mice. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 11th Meeting of Hirosaki International Forum of Medical Science | |||||
