@misc{oai:repo.qst.go.jp:00069726,
 author = {Takuwa, Hiroyuki and Masamoto, Kazuto and Obata, Takayuki and Kanno, Iwao and 田桑 弘之 and 正本 和人 and 小畠 隆行 and 菅野 巖},
 note = {The mechanism regulating cerebral blood flow (CBF) during brain functions (neurovascular coupling) was widely investigated with animals under the anesthetized condition. However, anesthesia is known to greatly affect neurovascular coupling and systemic physiology. Therefore, the present study aimed to develop a novel model for neurovascular coupling studies in awake-behaving mice. The male C57BL/6J mice (5-7 weeks) were initially anesthetized with isoflurane (2.5%) for the preparation of head attachment to apparatus. The skin of the head was cut and a metal head plate was attached to the skull. The animal was tethered by screwing the head plate onto apparatus, and then the anesthesia was discontinued for recovery of the animal. The regional CBF in the somatosensory barrel cortex was continuously measured with laser-Doppler flowmetry (LDF). Behavior of animal was also recorded using a digital camera. Whisker stimulation (frequency 10 Hz and duration 10 sec) was induced to the contralateral side of LDF recording site. During experiments, the animals showed no signs of struggling against the head restraint. Based on the type of the animal behavior, the experiment period was separated into three phases (grooming, running and resting). The CBF was observed to be stable during both resting and running phases, showing negligible motion artifacts. The increase in CBF was observed during grooming and whisker stimulation, which was higher than that in the anesthetized condition. In conclusion, the present model allows for the dynamic study of neurovascular coupling in awake-behaving mice., The 11th Meeting of Hirosaki International Forum of Medical Science},
 title = {Neurovascular coupling studies in awake-behaving mice.}
}