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Expressions of CD180 on Spleen cells induced Apoptosis by X-ray-irradiation
https://repo.qst.go.jp/records/69612
https://repo.qst.go.jp/records/6961299139dd5-02da-4c68-acb9-be78b5e7ae1d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-12-01 | |||||
| タイトル | ||||||
| タイトル | Expressions of CD180 on Spleen cells induced Apoptosis by X-ray-irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujita, Kazuko
× Fujita, Kazuko× Bing, Wang× Tanaka, Kaoru× Akasaka, Ki× Isii, Yosiharu× et.al× 藤田 和子× 王 冰× 田中 薫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The CD180, a cell surface receptor of lipopolysaccharide (LPS) and a homologue of Toll-like 4(TLR4) on B lymphocyte, has been revealed as a suppressing molecule of radiation-induced apoptosis in vitro and as a physiological negative regulator of TLR4 responses. However, few studies regarding the functional mechanism of the CD180 molecule associated with radiation-induced apoptosis have been reported in vivo. In the present study, we have examined the effect of irradiation on the expression patterns of CD180 and apoptosis of spleen cells associated with the expression of CD19 and CD22, B-cell signal transduction molecules, in vivo. BALB/c female mice (7-8 weeks) were irradiated with X-ray machine operated at 150 kVp and 20 mA at a dose rate of 5.4 Gy/m. Spleens were removed after whole body-irradiation. Histochemical analysis demonstrated that the number of CD180-positive B lymphocytes was significantly increased at 6 h after irradiation compared with that in both 3 h and 0 h (sham irradiation), whereas no significant change of the numbers of B lymphocytes positive for CD19 or CD22 was detected at 0 h, 3 h, and 6 h after irradiation. Flow cytometric analysis of the spleen cells showed that the percentage of CD180-presenting cells increased at 6 h after irradiation than that at 0 h. The expression of CD180 was increased dependently on radiation dose from 0 Gy to 10 Gy with histochemical analysis. To clarify the relationship of the expression patterns between apoptosis and CD180, double staining for TUNEL and CD180 was performed and showed that the number of CD180-positive cells positive for TUNEL was significantly less than that of CD180-negative cells positive for TUNEL at 6 h after irradiation. These results suggested that CD180-positive B lymphocytes were resistant to apoptosis induced by irradiation in vivo. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第38回日本免疫学会総会・学術集会 | |||||
| 発表年月日 | ||||||
| 日付 | 2008-12-03 | |||||
| 日付タイプ | Issued | |||||
