@misc{oai:repo.qst.go.jp:00069612,
 author = {Fujita, Kazuko and Bing, Wang and Tanaka, Kaoru and Akasaka, Ki and Isii, Yosiharu and et.al and 藤田 和子 and 王 冰 and 田中 薫},
 month = {Dec},
 note = {The CD180, a cell surface receptor of lipopolysaccharide (LPS) and a
homologue of Toll-like 4(TLR4) on B lymphocyte, has been revealed as a
suppressing molecule of radiation-induced apoptosis in vitro and as a
physiological negative regulator of TLR4 responses.  However, few studies
regarding the functional mechanism of the CD180 molecule associated with
radiation-induced apoptosis have been reported in vivo. In the present
study, we have examined the effect of irradiation on the expression patterns
of CD180 and apoptosis of spleen cells associated with the expression of
CD19 and CD22, B-cell signal transduction molecules, in vivo. BALB/c female
mice (7-8 weeks) were irradiated with X-ray machine operated at 150 kVp and
20 mA at a dose rate of 5.4 Gy/m. Spleens were removed after whole
body-irradiation. Histochemical analysis demonstrated that the number of
CD180-positive B lymphocytes was significantly increased at 6 h after
irradiation compared with that in both 3 h and 0 h (sham irradiation),
whereas no significant change of the numbers of B lymphocytes positive for
CD19 or CD22 was detected at 0 h, 3 h, and 6 h after irradiation.  Flow
cytometric analysis of the spleen cells showed that the percentage of
CD180-presenting cells increased at 6 h after irradiation than that at 0 h.
The expression of CD180 was increased dependently on radiation dose from 0
Gy to 10 Gy with histochemical analysis. To clarify the relationship of the
expression patterns between apoptosis and CD180, double staining for TUNEL
and CD180 was performed and showed that the number of CD180-positive cells
positive for TUNEL was significantly less than that of CD180-negative cells
positive for TUNEL at 6 h after irradiation. These results suggested that
CD180-positive B lymphocytes were resistant to apoptosis induced by
irradiation in vivo., 第38回日本免疫学会総会・学術集会},
 title = {Expressions of CD180 on Spleen cells induced Apoptosis by X-ray-irradiation},
 year = {2008}
}