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Evaluation of Antioxidative Ability of Antioxidative Food Factors by EPR Spin-Trapping, Stopped-Flow Kinetic Analysis, and Mitochondrial ROS Assay
https://repo.qst.go.jp/records/69507
https://repo.qst.go.jp/records/695070bab21f9-52de-491a-8107-a1f9fcfd5ebf
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-10-08 | |||||
| タイトル | ||||||
| タイトル | Evaluation of Antioxidative Ability of Antioxidative Food Factors by EPR Spin-Trapping, Stopped-Flow Kinetic Analysis, and Mitochondrial ROS Assay | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakanishi, Ikuo
× Nakanishi, Ikuo× Indo, Hiroko× Ikota, Nobuo× Nyuui, Minako× Matsumoto, Kenichiro× Ohkubo, Kei× Fukuzumi, Shunichi× Anzai, Kazunori× Ozawa, Toshihiko× Majima, Hideyuki× 中西 郁夫× 乳井 美奈子× 松本 謙一郎× 安西 和紀× 小澤 俊彦× 馬嶋 秀行 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Antioxidative food factors have attracted considerable interest with regard to the prevention against oxidative stress and associated diseases. However, evaluation and comparison of the antioxidative ability and function have scarcely been performed. In this study, we examined the antioxidative ability of eleven food factors, which are astaxanthin, canthaxanthin, zeaxanthin, lycopene, coenzyme Q10, sesamin, sesamol, catechin gallate, catechin hydrate, beta-carotene, and alpha-tocopherol, by the EPR spin-trapping of superoxide anion with DMPO, stopped-flow kinetic analysis with 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical, and a newly-developed mitochondrial ROS scavenging assay. Among eleven food factors, catechin gallate and catechin hydrate efficiently scavenged superoxide anion, while coenzyme Q10 and sesamin showed no scavenging activity against superoxide anion. Furthermore, catechin gallate and catechin hydrate also scavenged DPPH radical much faster than sesamol and alpha-tocopherol in deaerated ethanol, while DPPH radical was not scavenged by the rest of the food factors. On the other hand, no different effectiveness was observed for all food factors using mitochondrial ROS scavenging assay, suggesting that the evaluation of antioxidative ability by only one method is not enough to evaluate the in vivo effects. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Biomedical Redox Navigation (EPR2008: A Joint Conference of the 13th In Vivo ESR/EPR Spectroscopy & Imaging, the 10th International EPR Spin Trapping/Spin Labeling, and the 7th JSPS Core-to-Core Seminar) | |||||
| 発表年月日 | ||||||
| 日付 | 2008-09-30 | |||||
| 日付タイプ | Issued | |||||
