@misc{oai:repo.qst.go.jp:00069507,
 author = {Nakanishi, Ikuo and Indo, Hiroko and Ikota, Nobuo and Nyuui, Minako and Matsumoto, Kenichiro and Ohkubo, Kei and Fukuzumi, Shunichi and Anzai, Kazunori and Ozawa, Toshihiko and Majima, Hideyuki and 中西 郁夫 and 乳井 美奈子 and 松本 謙一郎 and 安西 和紀 and 小澤 俊彦 and 馬嶋 秀行},
 month = {Sep},
 note = {Antioxidative food factors have attracted considerable interest with regard to the prevention against oxidative stress and associated diseases.  However, evaluation and comparison of the antioxidative ability and function have scarcely been performed.  In this study, we examined the antioxidative ability of eleven food factors, which are astaxanthin, canthaxanthin, zeaxanthin, lycopene, coenzyme Q10, sesamin, sesamol, catechin gallate, catechin hydrate, beta-carotene, and alpha-tocopherol, by the EPR spin-trapping of superoxide anion with DMPO, stopped-flow kinetic analysis with 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical, and a newly-developed mitochondrial ROS scavenging assay.
Among eleven food factors, catechin gallate and catechin hydrate efficiently scavenged superoxide anion, while coenzyme Q10 and sesamin showed no scavenging activity against superoxide anion.  Furthermore, catechin gallate and catechin hydrate also scavenged DPPH radical much faster than sesamol and alpha-tocopherol in deaerated ethanol, while DPPH radical was not scavenged by the rest of the food factors.  On the other hand, no different effectiveness was observed for all food factors using mitochondrial ROS scavenging assay, suggesting that the evaluation of antioxidative ability by only one method is not enough to evaluate the in vivo effects., Biomedical Redox Navigation (EPR2008: A Joint Conference of the 13th In Vivo ESR/EPR Spectroscopy & Imaging, the 10th International EPR Spin Trapping/Spin Labeling, and the 7th JSPS Core-to-Core Seminar)},
 title = {Evaluation of Antioxidative Ability of Antioxidative Food Factors by EPR Spin-Trapping, Stopped-Flow Kinetic Analysis, and Mitochondrial ROS Assay},
 year = {2008}
}