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Quantum Dots in Multimodal Imaging: Probe Design, Spectral Characteristics, and Application
https://repo.qst.go.jp/records/69497
https://repo.qst.go.jp/records/69497456b5c38-6c69-41d8-9618-3ca9b5a37fe0
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-10-06 | |||||
| タイトル | ||||||
| タイトル | Quantum Dots in Multimodal Imaging: Probe Design, Spectral Characteristics, and Application | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Zhelev, Zhivko
× Zhelev, Zhivko× Bakalova-Zheleva, Rumiana× Aoki, Ichio× Veselina, Gadjeva× Kanno, Iwao× et.al× Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ× バカロバ ルミアナ× 青木 伊知男× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Over the past few years, semiconductor quantum dots (QDs) have won recognition as an important source of fluorofores for use in bioimaging and biosensing, owing to their unique and attractive optical properties. The careful design of the architecture of water-soluble QDs enables the creation of nanobioprobes that are compatible with several imaging techniques, so-called multimodal imaging. This opens the door to the existing prospect of simultaneous application of QD probes in a variety of techniques including optical imaging, magnetic resonance imaging (MRI), positron emission tomography, computed tomography and X-ray imaging. In the present study, we describe two multimodal QD probes with combined fluorescent and paramagnetic properties (Figure 1) and their simultaneous application in optical imaging and MRI. Using fluorescent confocal microscopy, we observed that the probes were characterized with easy and comparatively fast intracellular delivery in viable cells without transfection techniques that are inherently toxic or keep a risk of artifacts. Both probes penetrated through cytoplasmic membrane via endocytosis. The probes were localized into the cytoplasm, uniformly distributed. Using MRI, we quantified the amount of multimodal QDs delivered into the cells or adsorbed on the cell surface. Two independent calibration curves were used, based on the relationship between the concentration of the probe and T1 (longitudinal relaxation time) or T2 (transverse relaxation time) values, respectively. It was found that approximately 58% of the probes were delivered into the cells and/or bound on the cell surface. The photosensitization and cytotoxicity were clarified on several cultured cell lines before and after laser or ultraviolet irradiation. No cytotoxicity or photosensitization was detected up to 500 nM concentration. Figure 1. Design of paramagnetic silica-shelled quantum dots. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2008 World Molecular Imaging Congress | |||||
| 発表年月日 | ||||||
| 日付 | 2008-09-13 | |||||
| 日付タイプ | Issued | |||||
