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放射線感受性株を用いた放射線誘導性アポトーシス制御のクロストークの解析
https://repo.qst.go.jp/records/69477
https://repo.qst.go.jp/records/6947752e4b6b3-17c7-49ba-a2a5-d82b1a68e707
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-09-26 | |||||
| タイトル | ||||||
| タイトル | 放射線感受性株を用いた放射線誘導性アポトーシス制御のクロストークの解析 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
中島, 徹夫
× 中島, 徹夫× 王, 冰× 根井, 充× 中島 徹夫× 王 冰× 根井 充 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To clarify network of radiation-induced apoptosis, analysis of cross-talk of the regulation was performed. Protein kinase cascades are known to regulate systems in radiation-induced apoptosis. In radiosensitive mouse thymic lymphomas, 3SBH5, Protein kinase C(PKC), a family of protein kinases, regulates the radiation-induced apoptosis. In this cell line, participants in the PKC-related radiation-induced apoptosis regulation were explored. In 3SBH5, after irradiation, a decreased protein was found by Western blot in immunoprecipitates with anti-PKC specific phospho-substrate antibody. The protein was analysed by LC-MS/MS, and Mascot analysis was tried for the identification. Some candidates were selected. Of them, HSC70 is reported to be related to PKC regulation and cell cycle regulation. Therefore, HSC70 is a candidate of the participants. XR223 is a radioresistant cell line, which is selected from 3SBH5. By comparative analysis by two-dimensional electrophoresis between the cell lines, a protein, which increased in 3SBH5, but decreased in XR223 after irradiation was detected. Furthermore, exploration of participants related to lipid mediator-related PKC cascades was performed. We demonstrated that alteration of PKCδ regulation in 3SBH5 apparently renders the cells radiosensitive. Lipid mediator-related PKCδ regulation might distinguish different radiosensitivity between the cell lines. Involvement of the proteins mentioned above in the radiation-induced apoptosis regulations will be discussed with other candidates. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第４５回日本生物物理学会年会 | |||||
| 発表年月日 | ||||||
| 日付 | 2007-12-23 | |||||
| 日付タイプ | Issued | |||||
