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In vivo optical and magnetic resonance imaging of electroporation-mediated transgene expression in experimental tumors
https://repo.qst.go.jp/records/69458
https://repo.qst.go.jp/records/6945852730867-95ad-4f00-a88e-1985323b1608
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-09-18 | |||||
| タイトル | ||||||
| タイトル | In vivo optical and magnetic resonance imaging of electroporation-mediated transgene expression in experimental tumors | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
U, Winn Aung
× U, Winn Aung× Hasegawa, Sumitaka× Koshikawa, Michiko× Obata, Takayuki× Ikehira, Hiroo× Furukawa, Takako× Aoki, Ichio× Saga, Tsuneo× Ｕ Ｗｉｎｎ Ａｕｎｇ× 長谷川 純崇× 越川 道子× 小畠 隆行× 池平 博夫× 古川 高子× 青木 伊知男× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In vivo electroporation (EP) is one of the efficient methods for effective gene transfer. Application of in vivo EP-mediated gene transfer (EGT) in anticancer gene therapy is highly expected since it could be performed repeatedly, safely and easily with low cost. Meanwhile, effective transfer, long-term expression and non-invasive monitoring are critical for optimal gene therapy. One of the major challenges in molecular imaging is to evaluate gene therapy by imaging transgene expression in vivo at high spatial resolution. Here we report the feasibility of in vivo optical and MRI of EP-mediated gene expression in tumor model. Initially, we observed the in vivo EGT level and its temporal change by means of optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual-reporter plasmid carrying a gene encoding ferritin heavy chain (FHC), a magnetic resonance imaging (MRI) reporter, and RFP gene to visualize the intratumoral transgene by dual modality. In cellular T2-weighted MRI, cells transfected with dual-reporter plasmid showed lower signal intensity and increased transverse relaxation rate compared to the control cells. Moreover, after conducting in vivo EGT in the experimental tumor, the plasmid-injected region in the tumor showed both fluorescent light emission in optical imaging and detectable low signal intensity in T2-weighted MRI. The correlative immunohistological finding validated that both reporter genes were expressed in this region. Thus, our strategy would be a platform technology to evaluate EP-mediated gene therapy without necessity to administer contrast agent or substrate. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | World Molecular Imaging Congress | |||||
| 発表年月日 | ||||||
| 日付 | 2008-09-13 | |||||
| 日付タイプ | Issued | |||||
