@misc{oai:repo.qst.go.jp:00069458,
 author = {U, Winn Aung and Hasegawa, Sumitaka and Koshikawa, Michiko and Obata, Takayuki and Ikehira, Hiroo and Furukawa, Takako and Aoki, Ichio and Saga, Tsuneo and Ｕ Ｗｉｎｎ Ａｕｎｇ and 長谷川 純崇 and 越川 道子 and 小畠 隆行 and 池平 博夫 and 古川 高子 and 青木 伊知男 and 佐賀 恒夫},
 month = {Sep},
 note = {In vivo electroporation (EP) is one of the efficient methods for effective gene transfer. Application of in vivo EP-mediated gene transfer (EGT) in anticancer gene therapy is highly expected since it could be performed repeatedly, safely and easily with low cost. Meanwhile, effective transfer, long-term expression and non-invasive monitoring are critical for optimal gene therapy. One of the major challenges in molecular imaging is to evaluate gene therapy by imaging transgene expression in vivo at high spatial resolution. Here we report the feasibility of in vivo optical and MRI of EP-mediated gene expression in tumor model. Initially, we observed the in vivo EGT level and its temporal change by means of optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual-reporter plasmid carrying a gene encoding ferritin heavy chain (FHC), a magnetic resonance imaging (MRI) reporter, and RFP gene to visualize the intratumoral transgene by dual modality. In cellular T2-weighted MRI, cells transfected with dual-reporter plasmid showed lower signal intensity and increased transverse relaxation rate compared to the control cells. Moreover, after conducting in vivo EGT in the experimental tumor, the plasmid-injected region in the tumor showed both fluorescent light emission in optical imaging and detectable low signal intensity in T2-weighted MRI. The correlative immunohistological finding validated that both reporter genes were expressed in this region. Thus, our strategy would be a platform technology to evaluate EP-mediated gene therapy without necessity to administer contrast agent or substrate., World Molecular Imaging Congress},
 title = {In vivo optical and magnetic resonance imaging of electroporation-mediated transgene expression in experimental tumors},
 year = {2008}
}