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Free radical reaction and oxygen consumption in an aqueous solution by an X-ray irradiation
https://repo.qst.go.jp/records/69300
https://repo.qst.go.jp/records/6930079dba92f-b737-4cf4-bc4f-4783fb53e49c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-03-18 | |||||
| タイトル | ||||||
| タイトル | Free radical reaction and oxygen consumption in an aqueous solution by an X-ray irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matsumoto, Kenichiro
× Matsumoto, Kenichiro× Matsumoto, Atsuko× Nyuui, Minako× Ueno, Megumi× Manda, Sushma× Anzai, Kazunori× 松本 謙一郎× 松本 厚子× 乳井 美奈子× 上野 恵美× Ｍａｎｄａ Ｓｕｓｈｍａ× 安西 和紀 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: High LET carbon ion beam cancer therapy at National Institute of Radiological Sciences (Chiba, Japan) began in 1994. Considering the expansion of heavy-ion cancer therapy, the quality and accuracy of the heavy-ion therapy must be improved and optimized. Detection of free radical reactions and geometry in a large sample irradiated by a heavy-ion beam is thus important to understand the effect of high LET irradiation in patients. Therefore, a new method to detect free radical generation, which can be translated to an imaging technique, is required. In this experiment, using an X-ray irradiation, the imaging-translational detection of H2O2 generation in an aqueous sample was tested. Method: LiPc crystal was put into a plastic microtube. The microtube was filled with mili-Q water or PBS, and sealed air-tightly without any bubble in the tube. The tube was irradiated by X-ray (Eeff = 80 keV) with dose of 256 Gy. After the irradiation, EPR spectra of LiPc in the tube were measured at L-band (1.2 GHz). The pO2 value in the tube was estimated from the linewidth of EPR spectrum of LiPc. A similar sample without LiPc was irradiated by X-ray (256 Gy), and then H2O2 concentration in the tube was measured. A water solution of TEMPOL (2 mM) was irradiated by X-ray (356 Gy), and the reduction of the EPR signal of TEMPOL was measured. Results and Discussion: By irradiating 256 Gy of X-ray, pO2 in the irradiated water was decreased by 30 mmHg, and generation of 60 mol/L H2O2 in the irradiated water was detected. When a water solution of TEMPOL (2 mM) was irradiated by 256 Gy of X-ray, EPR signal of TEMPOL in the sample was decreased. The H2O2 generated in the water during the irradiation can make the reduction of TEMPOL without hydrogen-donor, such as GSH, NADH, or NADPH. This experimental design will be translated to imaging experiment and applied to the high LET radiation such as heavy-ion beams in the future. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Oxygen Club of California 2008 World Congress | |||||
| 発表年月日 | ||||||
| 日付 | 2008-03-15 | |||||
| 日付タイプ | Issued | |||||
