WEKO3
アイテム
DNA二本鎖切断修復遺伝子を欠損したヒト細胞株間におけるDNA損傷応答の比較
https://repo.qst.go.jp/records/69228
https://repo.qst.go.jp/records/69228351be05a-969b-4727-bcaa-368be75ac7e5
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-12-17 | |||||
| タイトル | ||||||
| タイトル | DNA二本鎖切断修復遺伝子を欠損したヒト細胞株間におけるDNA損傷応答の比較 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
小野田, 眞
× 小野田, 眞× 森, 雅彦× 勝部, 孝則× 辻, 秀雄× 塩見, 尚子× 塩見, 忠博× 小野田 眞× 森 雅彦× 勝部 孝則× 辻 秀雄× 塩見 尚子× 塩見 忠博 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA double-strand breaks (DSBs) are seriously dangerous lesions to cells. Higher eukaryotes have invested in non-homologous end-joining (NHEJ), a major mechanism for DSBs repair. The aim of this study is to define the biological roles of NHEJ-related genes on DNA damage induced by ionizing radiation (IR) through the generation and characterization of NHEJ-related gene deficient human cell lines. We have produced 3 cell lines that bearing a disrupted XRCC4, Artemis and MDC1, respectively, by using gene targeting technique in human colon tumor cell line HCT116. Chromosomal aberrations induced by X-ray irradiation were significantly higher in all cell lines deficient in NHEJ-related gene than that in wild HCT116. The highest survival rate was exhibited in the wild HCT116 and the lowest was in XRCC4-/- cells after X-irradiation, while MDC1-/- and Artemis-/- cells showed intermediated radio-sensitivities between the wild and XRCC4-/- cells. Formation of g-H2AX foci increased in a dose-dependent manner of X-rays and the number of foci peaked at 30 - 60 min after X-irradiation in all cell lines. In the wild HCT116, the foci then disappeared gradually and returned to the basal level within 4 hr, while a number of foci still remained at 4 hr after X-irradiation in XRCC4-/- and MDC1-/- cells. Formation of 53BP1 foci might be dependent upon MDC1, since many g-H2AX foci did not colocalized to 53BP1 foci in the MDC1-/- cells after X-irradiation. These rersults suggest that deficienies of NHEJ-related genes cause a deterioration of g-H2AX foci-associated event, probably a DNA DSB repair process and in turn become a significant reason for the radio-sensitivity of theses cells. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第80回日本生化学会 | |||||
| 発表年月日 | ||||||
| 日付 | 2007-12-15 | |||||
| 日付タイプ | Issued | |||||
