{"created":"2023-05-15T14:50:37.361228+00:00","id":69228,"links":{},"metadata":{"_buckets":{"deposit":"886207f2-6571-43cc-8abe-bad573bfe8f1"},"_deposit":{"created_by":1,"id":"69228","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69228"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069228","sets":["10:28"]},"author_link":["679317","679311","679312","679318","679314","679315","679320","679321","679316","679319","679310","679313"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-12-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA double-strand breaks (DSBs) are seriously dangerous lesions to cells. Higher eukaryotes have invested in non-homologous end-joining (NHEJ), a major mechanism for DSBs repair. The aim of this study is to define the biological roles of NHEJ-related genes on DNA damage induced by ionizing radiation (IR) through the generation and characterization of NHEJ-related gene deficient human cell lines.\n We have produced 3 cell lines that bearing a disrupted XRCC4, Artemis and MDC1, respectively, by using gene targeting technique in human colon tumor cell line HCT116. Chromosomal aberrations induced by X-ray irradiation were significantly higher in all cell lines deficient in NHEJ-related gene than that in wild HCT116. The highest survival rate was exhibited in the wild HCT116 and the lowest was in XRCC4-/- cells after X-irradiation, while MDC1-/- and Artemis-/- cells showed intermediated radio-sensitivities between the wild and XRCC4-/- cells. Formation of g-H2AX foci increased in a dose-dependent manner of X-rays and the number of foci peaked at 30 - 60 min after X-irradiation in all cell lines. In the wild HCT116, the foci then disappeared gradually and returned to the basal level within 4 hr, while a number of foci still remained at 4 hr after X-irradiation in XRCC4-/- and MDC1-/- cells. Formation of 53BP1 foci might be dependent upon MDC1, since many g-H2AX foci did not colocalized to 53BP1 foci in the MDC1-/- cells after X-irradiation. These rersults suggest that deficienies of NHEJ-related genes cause a deterioration of g-H2AX foci-associated event, probably a DNA DSB repair process and in turn become a significant reason for the radio-sensitivity of theses cells.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第80回日本生化学会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小野田, 眞"}],"nameIdentifiers":[{"nameIdentifier":"679310","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森, 雅彦"}],"nameIdentifiers":[{"nameIdentifier":"679311","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"勝部, 孝則"}],"nameIdentifiers":[{"nameIdentifier":"679312","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 秀雄"}],"nameIdentifiers":[{"nameIdentifier":"679313","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見, 尚子"}],"nameIdentifiers":[{"nameIdentifier":"679314","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見, 忠博"}],"nameIdentifiers":[{"nameIdentifier":"679315","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野田 眞","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森 雅彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679317","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"勝部 孝則","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679318","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 秀雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679319","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 尚子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 忠博","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679321","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"DNA二本鎖切断修復遺伝子を欠損したヒト細胞株間におけるDNA損傷応答の比較","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA二本鎖切断修復遺伝子を欠損したヒト細胞株間におけるDNA損傷応答の比較"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-17"},"publish_date":"2007-12-17","publish_status":"0","recid":"69228","relation_version_is_last":true,"title":["DNA二本鎖切断修復遺伝子を欠損したヒト細胞株間におけるDNA損傷応答の比較"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:16:58.651565+00:00"}