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Whole genome-wide screening of cervical lymph node metastasis-associated genetic alterations in oral squamous cell carcinoma of Japanese patients
https://repo.qst.go.jp/records/69109
https://repo.qst.go.jp/records/6910979ba1549-79d8-42ae-a418-ff9f51f405d2
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-10-01 | |||||
| タイトル | ||||||
| タイトル | Whole genome-wide screening of cervical lymph node metastasis-associated genetic alterations in oral squamous cell carcinoma of Japanese patients | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sugahara, Keisuke
× Sugahara, Keisuke× Michikawa, Yuichi× Ishikawa, Kenichi× Ootsuka, Yoshimi× Iwakawa, Mayumi× Shibahara, Takahiko× Imai, Takashi× 菅原 圭亮× 道川 祐市× 石川 顕一× 荘司 好美× 岩川 眞由美× 柴原 孝彦× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To precisely identify genetic markers that reflect occurrence of cervical lymph node metastasis (LNM) in Japanese oral squamous cell carcinoma (OSCC) patients, oligonucleotide array-based comparative genomic hybridization (CGH) with individual gene-level resolution and real-time quantitative polymerase chain reaction (QPCR) was conducted using primary tumor materials surgically resected from total of 54 OSCC patients with or without cervical LNM (LNM present, n = 22; LNM absent, n = 32). Frequent gain was observed at 11q13 region exclusively in the patient group with cervical LNM using array-based CGH. This observation was confirmed by real-time QPCR experiments designed for 11 genes (TPCN2, MYEOV, CCND1, ORAOV1, FGF4, TMEM16A, FADD, PPFIA1, CTTN, SHANK2 and DHCR7) in this region. Moreover, we refined a comprehensive physical map of the 11q13 amplification region including 33 kb of DNA sequence covering this region and found a novel breakpoint between MYEOV and CCND1. There were hypothetical two cores in 11q13 region. Combination of copy number amplification at CTTN and/or TPCN2/MYEOV, which were selected from each core separated by novel breakpoint, was most significantly associated with cervical LNM (P = 0.0035). Following detailed assessment of selected loci by real-time QPCR used to be quite adequate strategy for finding out genetic markers in regions with complicated alterations. Further study with larger patient numbers should be conducted to validate this result. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 14th European Cancer Conference, ESTRO26 meeting, European Society for therapeutic radiology and oncology | |||||
| 発表年月日 | ||||||
| 日付 | 2007-09-27 | |||||
| 日付タイプ | Issued | |||||
