@misc{oai:repo.qst.go.jp:00069109,
 author = {Sugahara, Keisuke and Michikawa, Yuichi and Ishikawa, Kenichi and Ootsuka, Yoshimi and Iwakawa, Mayumi and Shibahara, Takahiko and Imai, Takashi and 菅原 圭亮 and 道川 祐市 and 石川 顕一 and 荘司 好美 and 岩川 眞由美 and 柴原 孝彦 and 今井 高志},
 month = {Sep},
 note = {To precisely identify genetic markers that reflect occurrence of cervical lymph node metastasis (LNM) in Japanese oral squamous cell carcinoma (OSCC) patients, oligonucleotide array-based comparative genomic hybridization (CGH) with individual gene-level resolution and real-time quantitative polymerase chain reaction (QPCR) was conducted using primary tumor materials surgically resected from total of 54 OSCC patients with or without cervical LNM (LNM present, n = 22; LNM absent, n = 32). Frequent gain was observed at 11q13 region exclusively in the patient group with cervical LNM using array-based CGH. This observation was confirmed by real-time QPCR experiments designed for 11 genes (TPCN2, MYEOV, CCND1, ORAOV1, FGF4, TMEM16A, FADD, PPFIA1, CTTN, SHANK2 and DHCR7) in this region. Moreover, we refined a comprehensive physical map of the 11q13 amplification region including 33 kb of DNA sequence covering this region and found a novel breakpoint between MYEOV and CCND1. There were hypothetical two cores in 11q13 region. Combination of copy number amplification at CTTN and/or TPCN2/MYEOV, which were selected from each core separated by novel breakpoint, was most significantly associated with cervical LNM (P = 0.0035). Following detailed assessment of selected loci by real-time QPCR used to be quite adequate strategy for finding out genetic markers in regions with complicated alterations. Further study with larger patient numbers should be conducted to validate this result., The 14th European Cancer Conference, ESTRO26 meeting, European Society for therapeutic radiology and oncology},
 title = {Whole genome-wide screening of cervical lymph node metastasis-associated genetic alterations in oral squamous cell carcinoma of Japanese patients},
 year = {2007}
}