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Development of a risk assessment system of toxicants by HiCEP
https://repo.qst.go.jp/records/69032
https://repo.qst.go.jp/records/6903232a2fa78-0c2b-4e61-a1b0-d7cf7b80e735
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-18 | |||||
| タイトル | ||||||
| タイトル | Development of a risk assessment system of toxicants by HiCEP | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suetomi, Katsutoshi
× Suetomi, Katsutoshi× Fujimori, Akira× Kubota, Yoshihisa× Takahashi, Sentaro× 末冨 勝敏× 藤森 亮× 久保田 善久× 高橋 千太郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In our previous study, we have indicated a novel expression profiling method called 'high coverage expression profiling' (HiCEP) could detect an alteration in gene expression as small as 1.5-fold and covers 70% to 80% of all transcripts. As HiCEP has a great advantage over other common methods for detecting minimal alterations in gene expression as described above, our comprehensive approach using HiCEP method is thought to be a powerful strategy to develop the system of risk assessment of many chemicals. Arsenic is one of the typical toxic metals and is also known to be carcinogen. Exposure to arsenic is supposed to increase the risk of occurrences of tumors (lung, skin, liver, bladder, and kidney). In order to investigate the efficiency of HiCEP method on developing the system of risk assessment of arsenic in the environment, we carried out HiCEP analysis of human lung embryonic fibroblasts (HFLIII) after 1 and 2 hours' exposure to 1 micro M sodium arsenite. The most up-regulated gene is hemoxigenase 1 (HMOX1), which is known to be induced by arsenic. We examined the effect of arsenic on the HMOX1 expression by quantitative PCR (qPCR) after several periods of incubation time with 1-10 micro M sodium arsenite. HMOX1 expression reached to the peak value after 4 hours' incubation with 1-10 micro M arsenic. We also examined the effect of low level of arsenic on HMOX1 expression. HMOX1 expression reached to the peak value after 2 hours' incubation with arsenic at 0.1 and 0.3 micro M, and after 4 hours' incubation with arsenic at 0.5 micro M. Up-regulation of HMOX1 gene was observed in HFLIII cells treated with arsenic at 0.3 and 0.5 micro M. We examined the effect of arsenic concentration on cell viability. Arsenic at less than 1 micro M does hardly affect the viability of HFLIII cells. We further explored the effect of ionizing radiation on HMOX1 expression by qPCR 0.5, 2, and 4 hours after X-irradiation at 2 and 4 Gy. At all of the period of time after X-irradiation at 2 and 4 Gy, HMOX1 expression was not up-regulated. In HiCEP analysis, HMOX1 expression after low-dose X-irradiation was not upregulated as well. We conclude HiCEP could be used to develop the system of risk assessment of many chemicals and HMOX1 could be a good biomarker for arsenic contamination in the environment. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 13th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-12 | |||||
| 日付タイプ | Issued | |||||
