{"created":"2023-05-15T14:50:28.726755+00:00","id":69032,"links":{},"metadata":{"_buckets":{"deposit":"010e0b50-3345-41d4-a20c-de7917640eed"},"_deposit":{"created_by":1,"id":"69032","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69032"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069032","sets":["10:28"]},"author_link":["677520","677519","677517","677515","677516","677521","677518","677522"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-07-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In our previous study, we have indicated a novel expression\nprofiling method called 'high coverage expression profiling'\n(HiCEP) could detect an alteration in gene expression as small as\n1.5-fold and covers 70% to 80% of all transcripts. As HiCEP has a\ngreat advantage over other common methods for detecting minimal\nalterations in gene expression as described above, our comprehensive\napproach using HiCEP method is thought to be a powerful\nstrategy to develop the system of risk assessment of many\nchemicals.\nArsenic is one of the typical toxic metals and is also known to\nbe carcinogen. Exposure to arsenic is supposed to increase the risk\nof occurrences of tumors (lung, skin, liver, bladder, and kidney). In\norder to investigate the efficiency of HiCEP method on developing\nthe system of risk assessment of arsenic in the environment, we\ncarried out HiCEP analysis of human lung embryonic fibroblasts\n(HFLIII) after 1 and 2 hours' exposure to 1 micro M sodium arsenite.\nThe most up-regulated gene is hemoxigenase 1 (HMOX1), which is\nknown to be induced by arsenic. We examined the effect of arsenic\non the HMOX1 expression by quantitative PCR (qPCR) after\nseveral periods of incubation time with 1-10 micro M sodium arsenite.\nHMOX1 expression reached to the peak value after 4 hours'\nincubation with 1-10 micro M arsenic. We also examined the effect of\nlow level of arsenic on HMOX1 expression. HMOX1 expression\nreached to the peak value after 2 hours' incubation with arsenic at\n0.1 and 0.3 micro M, and after 4 hours' incubation with arsenic at 0.5\nmicro M. Up-regulation of HMOX1 gene was observed in HFLIII cells\ntreated with arsenic at 0.3 and 0.5 micro M. We examined the effect of\narsenic concentration on cell viability. Arsenic at less than 1 micro M\ndoes hardly affect the viability of HFLIII cells.\nWe further explored the effect of ionizing radiation on\nHMOX1 expression by qPCR 0.5, 2, and 4 hours after X-irradiation\nat 2 and 4 Gy. At all of the period of time after X-irradiation at 2 and\n4 Gy, HMOX1 expression was not up-regulated. In HiCEP analysis,\nHMOX1 expression after low-dose X-irradiation was not upregulated\nas well.\nWe conclude HiCEP could be used to develop the system of\nrisk assessment of many chemicals and HMOX1 could be a good\nbiomarker for arsenic contamination in the environment.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"13th International Congress of Radiation Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suetomi, Katsutoshi"}],"nameIdentifiers":[{"nameIdentifier":"677515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"677516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubota, Yoshihisa"}],"nameIdentifiers":[{"nameIdentifier":"677517","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Sentaro"}],"nameIdentifiers":[{"nameIdentifier":"677518","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"末冨 勝敏","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677519","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677520","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保田 善久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677521","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 千太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677522","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Development of a risk assessment system of toxicants by HiCEP","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a risk assessment system of toxicants by HiCEP"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-07-18"},"publish_date":"2007-07-18","publish_status":"0","recid":"69032","relation_version_is_last":true,"title":["Development of a risk assessment system of toxicants by HiCEP"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:19:17.825905+00:00"}