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Radio-sensitization by 17AAG in human tumor cells
https://repo.qst.go.jp/records/69026
https://repo.qst.go.jp/records/6902646ec66b9-e236-40a7-ba79-5dc2dd0fe32c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-17 | |||||
| タイトル | ||||||
| タイトル | Radio-sensitization by 17AAG in human tumor cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yu, Dong
× Yu, Dong× Sekine, Emiko× Ninomiya, Yasuharu× Fujimori, Akira× Okayasu, Ryuichi× et.al× 于 冬× 関根 絵美子× 二宮 康晴× 藤森 亮× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Heat-shock protein 90 (Hsp90) is a ubiquitous molecular chaperone protein, which is related to the stabilization and activation of various cell cycle checkpoints and signal transduction proteins. An effective Hsp90 inhibitor 17-AAG has now entered clinical trials. Insulin-like growth factor I receptor (IGF-IR) over-expressed HeLa cells (HeLa-IGF-IR) exhibit a radioresistant phenotype and are difficult to be treated with radio-therapy. We studied the radio-sensitization effects of 17-AAG in HeLa-IGF-IR cells. After pretreatment with 17-AAG for 24 hours, characteristics of irradiated cells were examined by colony-forming assay, MTT assay, western blotting, and immunostaining. 150nM of 17-AAG pretreatment significantly radio-sensitized HeLa-IGF-IR cells, but this sensitization was not observed with non-transfected HeLa cells. The protein expression of PARP after irradiation was reduced with 17-AAG pretreatment, indicating an increase in apoptosis formation. Irradiation with 17-AAG pretreatment in HeLa-IGF-IR cells led to reduction in cell growth kinetics when compared to HeLa cells. The downstream pathways of IGF-IR (PI3-K/Akt and Raf/MEK/ERK pathways, proliferation signal pathways) were inhibited by 17-AAG treatment, and more cells were led to apoptosis. We also used the tumor xenografts model using SQ5 lung carcinoma cells to examine the antitumor effect of 17-AAG in vivo. The combined treatment with 17-AAG (80mg/kg, 1-3 times/week, i.p.) and 8 Gy gamma-rays significantly inhibited tumor growth. 17AAG treatment would be a useful method for certain radio-resistant tumor cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 13th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-12 | |||||
| 日付タイプ | Issued | |||||
