{"created":"2023-05-15T14:50:28.467171+00:00","id":69026,"links":{},"metadata":{"_buckets":{"deposit":"3f311aec-ab60-4a9c-a242-f8f747834202"},"_deposit":{"created_by":1,"id":"69026","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69026"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069026","sets":["10:28"]},"author_link":["677450","677447","677442","677444","677452","677451","677448","677449","677443","677445","677446"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-07-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Heat-shock protein 90 (Hsp90) is a ubiquitous molecular chaperone protein, which is related to the stabilization and activation of various cell cycle checkpoints and signal transduction proteins. An effective Hsp90 inhibitor 17-AAG has now entered clinical trials. Insulin-like growth factor I receptor (IGF-IR) over-expressed HeLa cells (HeLa-IGF-IR) exhibit a radioresistant phenotype and are difficult to be treated with radio-therapy. We studied the radio-sensitization effects of 17-AAG in HeLa-IGF-IR cells. After pretreatment with 17-AAG for 24 hours, characteristics of irradiated cells were examined by colony-forming assay, MTT assay, western blotting, and immunostaining. 150nM of 17-AAG pretreatment significantly radio-sensitized HeLa-IGF-IR cells, but this sensitization was not observed with non-transfected HeLa cells. The protein expression of PARP after irradiation was reduced with 17-AAG pretreatment, indicating an increase in apoptosis formation. Irradiation with 17-AAG pretreatment in HeLa-IGF-IR cells led to reduction in cell growth kinetics when compared to HeLa cells. The downstream pathways of IGF-IR (PI3-K/Akt and Raf/MEK/ERK pathways, proliferation signal pathways) were inhibited by 17-AAG treatment, and more cells were led to apoptosis. We also used the tumor xenografts model using SQ5 lung carcinoma cells to examine the antitumor effect of 17-AAG in vivo. The combined treatment with 17-AAG (80mg/kg, 1-3 times/week, i.p.) and 8 Gy gamma-rays significantly inhibited tumor growth. 17AAG treatment would be a useful method for certain radio-resistant tumor cells.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"13th International Congress of Radiation Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yu, Dong"}],"nameIdentifiers":[{"nameIdentifier":"677442","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sekine, Emiko"}],"nameIdentifiers":[{"nameIdentifier":"677443","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ninomiya, Yasuharu"}],"nameIdentifiers":[{"nameIdentifier":"677444","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"677445","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"677446","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"677447","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"于 冬","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677448","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"関根 絵美子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677449","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"二宮 康晴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677450","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677451","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677452","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Radio-sensitization by 17AAG in human tumor cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radio-sensitization by 17AAG in human tumor cells"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-07-17"},"publish_date":"2007-07-17","publish_status":"0","recid":"69026","relation_version_is_last":true,"title":["Radio-sensitization by 17AAG in human tumor cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:19:22.307335+00:00"}