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The effect of heavy-ions on synchronously dividing
https://repo.qst.go.jp/records/69010
https://repo.qst.go.jp/records/69010a3e92b20-abdb-42c6-b81e-cc2a80136f50
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-07-17 | |||||
| タイトル | ||||||
| タイトル | The effect of heavy-ions on synchronously dividing | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kato, Takamitsu
× Kato, Takamitsu× et.al× 加藤 宝光 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We investigated the alteration of homologous recombination repair in synchronous CHO cells irradiated with heavy ion particles (290MeV/n carbon ion, LET: 13keV/lm and 70keV/lm) and Xrays. The synchronized cell populations were prepared by mitotic shake-off and sequential harvesting at various time points. Our flow cytometry analysis revealed that the majority of cells enter S-phase at 8 hours, and G2-phase at 12 hours after plating. We scored the number of co-localization sites of gamma-H2AX (DNA double strand breaks) and Rad51 (homologous recombination repair protein) foci after 1Gy irradiation. Carbon irradiated cells showed a significantly higher number of co-localization sites at 30 minutes after irradiation than X-irradiated cells. We also observed delayed disappearance of Rad51 foci in carbon irradiated cells up to 2 hours. Both chromosome- and chromatid-type aberrations were the highest in high LET carbon(70keV/lm), intermediate in low LET carbon (13keV/lm), and the lowest in x-irradiated cells. Furthermore, we observed a significantly increased number of chromatid-type aberrations in cells irradiated at early S-phase by 70 keV/lm carbon ions. The cell survival rates by colony formation seem to reflect the chromosomal data as expected. We did not observe the resistant peak at late-S phase in cells irradiated with high LET (70 keV/lm) carbon ions. These results may indicate the alteration of homologous recombination repair after high LET carbon irradiation lead to various biological effects. Our data are consistent with the idea of complex type DSB induced by high LET heavy ion irradiation. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2007-07-13 | |||||
| 日付タイプ | Issued | |||||
